FastQCFastQC Report
Wed 25 May 2016
SRR1780869_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780869_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354197
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGT125643.5471785475314586No Hit
TCTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCG21680.612088752869166No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17550.4954869747626321No Hit
ATTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGT16430.46386615358119915No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCC11410.32213711578584797No Hit
CTTAAACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGT5400.15245753069619447No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT150.002225872469.9822133
AGGGGGG19000.060.966870
ACGTATG200.00693179952.5311640
CGTCTTC24050.051.5045848
ATCTCGT24400.050.93795837
GCCGTCT24100.050.8313146
CCGTCTT24000.050.7514347
TCGTATG25800.050.49506840
TATGCCG24700.050.46101843
CGTATGC24850.050.45245741
TCTCGTA25200.050.43946538
CTCGTAT25400.050.32512739
GTCTTCT24450.050.23262449
ATGCCGT25300.049.67946644
CGTATGA1200.049.61276241
TGCCGTC25250.049.49353445
CTTGAAA25700.049.44417657
GATCTCG25350.049.30525636
TCTTCTG24750.049.19961550
GACGATC24350.049.14561533
Produced by FastQC (version 0.11.5)