FastQCFastQC Report
Wed 25 May 2016
SRR1780868_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780868_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106682
Sequences flagged as poor quality0
Sequence length76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2803626.279972253988486No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG15501.4529161433044No Hit
TCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT2100.1968467032864026No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC1340.12560694400179973Illumina Single End PCR Primer 1 (95% over 21bp)
CTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG1160.1087343694343938No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAAA150.002220184369.9934462
GCCGCGA252.3822843E-455.9947478
TGTAAAC200.006924552452.519749
ACAGTGT200.00693741452.4950837
CGTGCCA200.00693741452.4950827
TCCAGCC200.00693741452.495088
TTTGCCC200.00693741452.4950832
ATAGTTC200.00693741452.4950841
CGATCAA200.00693741452.4950831
GAAACCC305.858018E-446.66229228
GTAGATC2800.046.24566738
CTCGGTG2900.044.6719344
AGATCTC2900.044.65098640
GATCTCG2900.044.65098641
ATCTCGG2950.043.8941942
TCTCGGT3000.043.18286543
TAGATCT3000.043.1626239
TCGGTGG3050.042.4749545
GTCGCCG2900.042.2374251
GCCGTAT2900.042.2374254
Produced by FastQC (version 0.11.5)