FastQCFastQC Report
Wed 25 May 2016
SRR1780868_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780868_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106682
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGT1558214.60602538385107No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCG27542.581503908813108No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26722.5046399580060363No Hit
ATTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGT16521.548527399186367No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCC14411.3507433306462198No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGT6510.610224780187848No Hit
ATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGTCTT4940.4630584353499185Illumina PCR Primer Index 4 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCC3550.33276466507939484No Hit
CTTATACACATCTCCGCGCCCACGAGACTTCTACCAATCTCGTATGCCGT2970.27839748036219797No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTACCACTCTCGTATGCCGT1830.1715378414352937No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTACCAATATCGTATGCCGT1750.1640389194053355No Hit
CTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGTC1180.11060909994188337No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGACGT1110.10404754316566994No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGATC150.002216468370.0229911
GGGTATC150.002216468370.022999
ATCATGG150.002216468370.022998
AACGTAT150.002220597169.9901639
TAACGTA150.002220597169.9901638
CCAAGCG150.002220597169.9901634
CCCGAGA252.8257073E-669.9901621
AGCCGTC600.064.1576445
AAGCCGT600.064.1576444
AGGGGGG22350.063.8836370
AAACTCG308.335241E-658.3251336
TCTGCTA1050.056.68527653
CTTAAAC2100.054.9922681
CCGTCTA700.054.99226447
CTGATTG452.665729E-854.4623254
AATATCG900.054.43678736
ATATCGT900.054.43678737
CGTATGA1050.053.32583641
CATGGAA200.00692583752.5172410
TGGAATA200.00692583752.5172412
Produced by FastQC (version 0.11.5)