##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780868_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 106682 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.26259350218406 32.0 27.0 32.0 13.0 32.0 2 30.01686320091487 32.0 32.0 32.0 27.0 32.0 3 29.586331339869893 32.0 32.0 32.0 22.0 32.0 4 30.45883091805553 32.0 32.0 32.0 27.0 32.0 5 29.449382276297783 32.0 32.0 32.0 22.0 32.0 6 33.61620517050674 37.0 32.0 37.0 22.0 37.0 7 34.03008005099267 37.0 37.0 37.0 27.0 37.0 8 33.31152396842954 37.0 32.0 37.0 22.0 37.0 9 33.774854239703046 37.0 37.0 37.0 22.0 37.0 10-11 33.68799328846478 37.0 34.5 37.0 24.5 37.0 12-13 34.01806771526593 37.0 37.0 37.0 27.0 37.0 14-15 33.81155677621342 37.0 37.0 37.0 27.0 37.0 16-17 32.66878667441556 37.0 29.5 37.0 20.0 37.0 18-19 33.46184454734632 37.0 32.0 37.0 22.0 37.0 20-21 33.68830730582479 37.0 34.5 37.0 24.5 37.0 22-23 33.0710757203652 37.0 32.0 37.0 17.5 37.0 24-25 33.59479574811121 37.0 34.5 37.0 24.5 37.0 26-27 33.76940814757879 37.0 34.5 37.0 24.5 37.0 28-29 33.78813201852233 37.0 37.0 37.0 24.5 37.0 30-31 33.90200783637352 37.0 37.0 37.0 27.0 37.0 32-33 33.03884441611518 37.0 34.5 37.0 17.5 37.0 34-35 33.15341857108041 37.0 32.0 37.0 22.0 37.0 36-37 31.864386681914475 37.0 29.5 37.0 13.0 37.0 38-39 31.93983989801466 37.0 32.0 37.0 13.0 37.0 40-41 32.963400573667535 37.0 32.0 37.0 17.5 37.0 42-43 33.514815057835435 37.0 37.0 37.0 22.0 37.0 44-45 33.26546183986052 37.0 32.0 37.0 22.0 37.0 46-47 33.172283046812026 37.0 32.0 37.0 22.0 37.0 48-49 32.73341332183499 37.0 32.0 37.0 13.0 37.0 50-51 32.923904688701 37.0 32.0 37.0 17.5 37.0 52-53 32.47213213100617 37.0 32.0 37.0 13.0 37.0 54-55 32.321347556288785 37.0 32.0 37.0 13.0 37.0 56-57 32.43646069627491 37.0 32.0 37.0 13.0 37.0 58-59 32.17536697849684 37.0 32.0 37.0 13.0 37.0 60-61 32.53456534373184 37.0 32.0 37.0 13.0 37.0 62-63 32.542345475337925 37.0 32.0 37.0 13.0 37.0 64-65 32.49228079713541 37.0 32.0 37.0 13.0 37.0 66-67 32.30849159183367 37.0 32.0 37.0 13.0 37.0 68-69 31.58043062559757 37.0 29.5 37.0 13.0 37.0 70-71 29.924340563543993 37.0 24.5 37.0 13.0 37.0 72-73 31.512063890815696 37.0 29.5 37.0 13.0 37.0 74-75 32.32926360585666 37.0 32.0 37.0 13.0 37.0 76 32.31217075045462 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 -0.7251986158764865 11101 2 -1.3188396879791284 11101 3 -1.1838779293277746 11101 4 0.21915960101546617 11101 5 -1.120324048330243 11101 6 -1.961469325323371 11101 7 -0.7426639413923368 11101 8 -0.6958051610428981 11101 9 0.10773420953579915 11101 10-11 -0.5647425341934138 11101 12-13 -0.8823289546223378 11101 14-15 0.34362005243986005 11101 16-17 -0.5572657784838739 11101 18-19 0.8442299620823235 11101 20-21 0.8902937923246625 11101 22-23 -0.46623395052451144 11101 24-25 -0.4174138891720034 11101 26-27 0.5222884572093136 11101 28-29 -0.4024231233845086 11101 30-31 -1.8842576327343323 11101 32-33 -1.1595058638912263 11101 34-35 -0.9459354309457666 11101 36-37 -0.8391219767998095 11101 38-39 -0.3822065157276704 11101 40-41 -0.9179437015221907 11101 42-43 -0.9564301345184276 11101 44-45 0.13810757313277833 11101 46-47 -0.8984653131762599 11101 48-49 -0.16252476675438032 11101 50-51 0.4851520475925355 11101 52-53 -1.3314859426726073 11101 54-55 -1.4081458758212726 11101 56-57 0.10797793238356945 11101 58-59 -1.674697025599599 11101 60-61 -0.3531911416891518 11101 62-63 -1.731032095811102 11101 64-65 -2.049562569557839 11101 66-67 -2.266258310065158 11101 68-69 -0.8990411962384073 11101 70-71 -1.7358699216114069 11101 72-73 -0.9624033961412408 11101 74-75 -1.4415552722870295 11101 76 -0.6778622192492847 11102 1 -0.3290633501760034 11102 2 -0.6648541807327497 11102 3 0.09329598371570214 11102 4 -0.4746809786946784 11102 5 -1.4548651111321753 11102 6 0.5542311577684131 11102 7 -0.8646446177208418 11102 8 0.109750394512659 11102 9 -0.7177488822516338 11102 10-11 -1.182194225014662 11102 12-13 -0.4822685681489105 11102 14-15 0.24096304760893617 11102 16-17 0.23258929397989192 11102 18-19 -0.13886182535729574 11102 20-21 0.9452454831459178 11102 22-23 0.8625703973015746 11102 24-25 -0.116085386756545 11102 26-27 -0.38048932056847207 11102 28-29 0.30772180415172556 11102 30-31 -0.15629869553626463 11102 32-33 0.6641656336933153 11102 34-35 -0.12196200099407406 11102 36-37 0.2635350280311073 11102 38-39 -0.5845012017180089 11102 40-41 -0.5767601266429665 11102 42-43 -0.4621668494942739 11102 44-45 -0.7507813157561074 11102 46-47 -0.7287793228380934 11102 48-49 -0.5985151049186328 11102 50-51 -0.8276498847746083 11102 52-53 -0.7828748315615002 11102 54-55 -1.8785565038405991 11102 56-57 -1.0765027439449355 11102 58-59 -1.6629216632807555 11102 60-61 0.3436687616924914 11102 62-63 -0.8071190523328369 11102 64-65 -0.5972678835675005 11102 66-67 -0.23636700571733016 11102 68-69 0.354883924534537 11102 70-71 -0.7959544626742101 11102 72-73 0.6103623043418551 11102 74-75 -0.3292364317073222 11102 76 0.10112328799709047 11103 1 1.2148835818306303 11103 2 -0.28487905635464017 11103 3 -1.994390583448478 11103 4 -1.238984461281742 11103 5 -1.0348816948801023 11103 6 0.11683480619296205 11103 7 -0.30204096929629287 11103 8 -0.19829272323195113 11103 9 0.9528564245476971 11103 10-11 -1.4930358502219647 11103 12-13 -1.3505314205535512 11103 14-15 -0.7942774167360014 11103 16-17 -0.12313209905492783 11103 18-19 -0.7524604986574559 11103 20-21 -1.0719601553283766 11103 22-23 -0.7017952743402205 11103 24-25 -0.6830422690119349 11103 26-27 -1.4578110452782553 11103 28-29 -1.4982483451020059 11103 30-31 -0.5848849310254849 11103 32-33 -0.08044464823039732 11103 34-35 -0.7451999777768279 11103 36-37 -2.046995651902556 11103 38-39 -0.9711098252669288 11103 40-41 -0.8738165113859182 11103 42-43 -0.9572953735407097 11103 44-45 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11104 24-25 -0.9633076089787664 11104 26-27 -1.096759161838314 11104 28-29 -2.2557702593403377 11104 30-31 -0.9449891717267391 11104 32-33 -0.5501200805924 11104 34-35 -0.6547000962321761 11104 36-37 -0.7880522735561897 11104 38-39 -2.007120249337053 11104 40-41 -1.570807745690587 11104 42-43 -0.760777960605381 11104 44-45 -1.3142733792481707 11104 46-47 -1.4371126561714291 11104 48-49 -0.2294674858710124 11104 50-51 -0.46653877366350116 11104 52-53 -2.2283113394980063 11104 54-55 -1.9360961863802828 11104 56-57 -2.478288458230651 11104 58-59 -2.3603422982013917 11104 60-61 -0.62855346052973 11104 62-63 -1.033309528523315 11104 64-65 -1.4484583597579785 11104 66-67 -0.48537494222527044 11104 68-69 -1.9476954405448232 11104 70-71 -1.6937719229916688 11104 72-73 -3.2636456321660816 11104 74-75 -0.6903475428184294 11104 76 -1.553638616764811 11105 1 -2.4598877229358607 11105 2 -0.2468434280445777 11105 3 -1.709123371123006 11105 4 0.21214697829456952 11105 5 -0.5471590179422137 11105 6 0.04437452694404698 11105 7 1.0609826224225287 11105 8 0.7826894626130212 11105 9 -0.2683761223950043 11105 10-11 -1.0556261246562428 11105 12-13 -0.11690118463636168 11105 14-15 0.5760884956376131 11105 16-17 -0.7620343619340844 11105 18-19 0.5833603968649328 11105 20-21 -0.5193602516211016 11105 22-23 -1.0910586349564895 11105 24-25 1.0375884483689006 11105 26-27 -0.02341046752187026 11105 28-29 0.2028830944743092 11105 30-31 0.7238536342128441 11105 32-33 0.1274272824388376 11105 34-35 -0.40444407984712427 11105 36-37 -1.7382570866642304 11105 38-39 -0.0813650010011635 11105 40-41 0.7892792998803344 11105 42-43 0.1451360178892429 11105 44-45 0.3773548849607451 11105 46-47 1.3164715732192533 11105 48-49 -0.1666154633415715 11105 50-51 -0.4925244367459385 11105 52-53 -0.7427762652532515 11105 54-55 -0.8754203031237537 11105 56-57 -0.10181636401662075 11105 58-59 -1.1232711256463475 11105 60-61 -0.24414485837919386 11105 62-63 -0.08400077276294837 11105 64-65 -3.0466406434241335 11105 66-67 -0.8687594788356137 11105 68-69 -1.9231178675801566 11105 70-71 0.5828537810533909 11105 72-73 1.0608551358830738 11105 74-75 -0.7781611628901146 11105 76 1.5876824277820418 11106 1 -0.667952239064892 11106 2 -0.38985418073275113 11106 3 0.351629317049035 11106 4 -0.783014312028012 11106 5 -0.9493095555766189 11106 6 -0.31799106445380687 11106 7 1.6797998267236025 11106 8 -1.1624718277095596 11106 9 0.5128066733039205 11106 10-11 0.8330835527631066 11106 12-13 0.23578698740665516 11106 14-15 1.1465186031645018 11106 16-17 0.30758929397989476 11106 18-19 -0.36663960313507005 11106 20-21 -0.1908656279651879 11106 22-23 0.5584037306349074 11106 24-25 0.7144701687990107 11106 26-27 0.3972884572093136 11106 28-29 0.6660551374850598 11106 30-31 0.4034235266859554 11106 32-33 0.1586100781377624 11106 34-35 0.39609355456147455 11106 36-37 0.2579794724755544 11106 38-39 0.4210543538375511 11106 40-41 0.20379542891258495 11106 42-43 0.3725553727279447 11106 44-45 0.13810757313277833 11106 46-47 -0.16766821172698343 11106 48-49 1.1487071173035943 11106 50-51 -0.530427662552384 11106 52-53 0.4351807239940584 11106 54-55 -1.0646676149517091 11106 56-57 -0.5195582995004919 11106 58-59 0.2662450033859045 11106 60-61 1.1964465394702657 11106 62-63 -0.16545238566617115 11106 64-65 -1.3111567724563926 11106 66-67 0.10113299428266487 11106 68-69 -1.1965049643543502 11106 70-71 0.538767759548012 11106 72-73 0.9589734154529665 11106 74-75 -0.3042364317073165 11106 76 0.8511232879970905 11107 1 -1.3338942680504005 11107 2 -0.47825997783419893 11107 3 -0.8360518423712548 11107 4 -0.47649257289757685 11107 5 -0.01887477296792639 11107 6 -0.5266867166277223 11107 7 -0.8151277095082747 11107 8 -0.17406603060810966 11107 9 -1.095164341188834 11107 10-11 -0.7893802153528355 11107 12-13 0.052453654073318035 11107 14-15 -1.228843715676085 11107 16-17 -0.7674107060201045 11107 18-19 0.06886764324174521 11107 20-21 -1.0444888163709933 11107 22-23 0.019273295852300976 11107 24-25 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0.3287431401337457 21611 18-19 -0.1474088339042936 21611 20-21 0.39503180793224857 21611 22-23 -0.3249296026984254 21611 24-25 0.16062401495285883 21611 26-27 0.2979294828503356 21611 28-29 1.000029496459419 21611 30-31 -0.3234995502371163 21611 32-33 -0.01318479365711056 21611 34-35 -1.0936500351821081 21611 36-37 -0.8433025788064974 21611 38-39 -1.756509748726554 21611 40-41 0.39225696737413074 21611 42-43 0.32255537272794754 21611 44-45 0.029133214158427734 21611 46-47 -0.826001545060322 21611 48-49 -0.06219031859384927 21611 50-51 -0.5092738163985402 21611 52-53 1.1621038009171372 21611 54-55 1.07764007735598 21611 56-57 0.5842878543456607 21611 58-59 1.5630398751807775 21611 60-61 0.8393952574189854 21611 62-63 -0.22378571899950117 21611 64-65 -0.5919260032256197 21611 66-67 -0.6706618775122024 21611 68-69 0.6054181125687315 21611 70-71 0.05287032365057698 21611 72-73 0.4698708513504002 21611 74-75 -1.18821079068168 21611 76 0.5177899546637619 21612 1 0.6198855987729459 21612 2 0.19212780124923157 21612 3 0.4277554431751618 21612 4 -0.8519332309469299 21612 5 1.4092490029819373 21612 6 1.1585855121227695 21612 7 -0.6927226957989134 21612 8 0.8672579020201638 21612 9 1.161004871502115 21612 10-11 0.4520024716820288 21612 12-13 0.14029149191115664 21612 14-15 0.6996717563176489 21612 16-17 0.676282987673595 21612 18-19 0.009036072540610007 21612 20-21 2.010936173836612 21612 22-23 0.5669622891934623 21612 24-25 0.17708278141162737 21612 26-27 -0.1376214527005999 21612 28-29 -0.36119711476719374 21612 30-31 0.9110811843436153 21612 32-33 1.367619087146771 21612 34-35 0.8902376987056186 21612 36-37 0.057078571574653125 21612 38-39 1.6230813808645799 21612 40-41 1.015957591074752 21612 42-43 0.8495823997549721 21612 44-45 0.8993688343940462 21612 46-47 0.44201647295770385 21612 48-49 1.346680090276557 21612 50-51 1.20538314825842 21612 52-53 1.3441897330030699 21612 54-55 1.1668639165798282 21612 56-57 0.7885498086076126 21612 58-59 1.799353111494021 21612 60-61 0.8489240169477412 21612 62-63 0.3392773440635608 21612 64-65 0.6726270113273927 21612 66-67 -0.3912093480596752 21612 68-69 0.798765305915925 21612 70-71 0.8040830748633248 21612 72-73 0.25244188392143485 21612 74-75 1.3013941989233118 21612 76 -0.08581364893984755 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 7.0 17 10.0 18 35.0 19 72.0 20 114.0 21 258.0 22 433.0 23 693.0 24 1019.0 25 1377.0 26 1972.0 27 2721.0 28 3513.0 29 4635.0 30 6478.0 31 8973.0 32 12950.0 33 18980.0 34 24022.0 35 16148.0 36 2271.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.578653265577827 21.123591291792458 12.503515910667742 36.79423953196197 2 15.793963254593177 17.54686914135733 47.1541057367829 19.50506186726659 3 14.706323466001761 20.61828612137005 46.52237490860689 18.153015504021297 4 10.966236103560114 36.84876548996082 32.91558088524775 19.269417521231322 5 10.891246883260532 32.39253107365816 45.71717815564013 10.99904388744118 6 21.026434195725535 51.87757780277465 17.01162354705662 10.084364454443193 7 20.360145858135155 26.025740773723037 18.32694344716392 35.28716992097788 8 18.57295513769896 49.650362760353204 14.58821544402992 17.188466657917925 9 19.016328902720232 16.791961155583884 14.960349449766596 49.23136049192928 10-11 19.456890571980278 32.73513807390188 29.76931441105341 18.038656943064435 12-13 19.36752409164198 17.979208069293936 32.22702388541003 30.42624395365405 14-15 18.661067471550965 17.985227123600982 18.782456271910913 44.57124913293714 16-17 31.49914461812472 30.282393194441187 17.197628365869093 21.020833821564995 18-19 32.470484573239034 22.280339140338295 17.880794701986755 27.368381584435912 20-21 18.987982526856523 19.443559121843304 17.72202328502597 43.84643506627421 22-23 18.535547400439626 32.41424239441702 17.708318686932596 31.34189151821075 24-25 31.589949334220723 32.033333489564534 17.619130198398008 18.757586977816732 26-27 32.19110023716453 33.64267836553334 18.8044283210064 15.361793076295735 28-29 18.657255078414277 21.903034393543123 30.6101596407848 28.8295508872578 30-31 17.809169392862835 19.269598147748894 34.660342516474344 28.26088994291392 32-33 17.3983755079467 30.90753143762391 32.712470530889256 18.981622523540135 34-35 17.572317729326407 22.66502315292177 19.87776757091168 39.88489154684014 36-37 17.78697437243396 42.9950694587653 18.584672203370765 20.633283965429968 38-39 17.925103112110985 23.57517810273716 31.751499812523438 26.748218972628422 40-41 17.65508190574395 18.225024020997868 33.64955121745447 30.470342855803707 42-43 31.062022806844862 17.812398936992928 32.00363709650961 19.1219411596526 44-45 18.21437108347902 31.091728026471817 33.93576085377228 16.758140036276885 46-47 30.02418470364364 18.947027999887514 23.40807469136381 27.620712605105034 48-49 29.399523818451094 17.676365272492077 20.563450254025984 32.360660655030834 50-51 19.379074705074544 18.137505331389818 33.41410486550837 29.06931509802727 52-53 15.846605236269557 18.274449516774624 45.92750213256592 19.9514431143899 54-55 15.50092568723489 18.630451594759904 35.86698225961426 30.001640458390945 56-57 28.674350909593745 18.1075012773218 23.369598335028616 29.848549478055848 58-59 17.00123265264036 19.048467151916235 43.694958310094165 20.255341885349242 60-61 29.00684289463817 30.24793775778028 23.17538432695913 17.569835020622424 62-63 16.650419002268425 47.0941677133912 18.314242327665397 17.94117095667498 64-65 15.559128343825144 48.848088271827734 17.469684683206697 18.123098701140428 66-67 15.820752815676864 48.76757015171612 17.185588608870496 18.22608842373652 68-69 16.64463817022872 48.345050618672666 16.974128233970752 18.03618297712786 70-71 27.729422407206904 37.12286326288604 16.859072608049647 18.28864172185741 72-73 45.38040803700735 18.778326139021292 17.288377085061608 18.552888738909743 74-75 46.99811107621784 17.55105484440986 17.303573018856426 18.147261060515866 76 47.005886984888825 17.089129701151148 17.164123139225318 18.74086017473471 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 1.5 15 4.5 16 12.5 17 17.0 18 14.5 19 27.0 20 47.0 21 52.0 22 83.0 23 121.5 24 146.5 25 164.0 26 235.0 27 365.5 28 501.0 29 577.0 30 683.0 31 869.0 32 1187.0 33 1425.0 34 1524.5 35 1808.5 36 2118.5 37 2244.0 38 2593.5 39 3369.0 40 4031.5 41 4531.5 42 4795.0 43 5249.0 44 6417.5 45 10433.0 46 13734.0 47 17767.5 48 13854.0 49 5281.5 50 4656.0 51 4080.0 52 3135.5 53 2571.0 54 2375.0 55 2251.0 56 1956.0 57 1455.0 58 1125.0 59 1000.5 60 827.0 61 676.0 62 574.0 63 529.5 64 430.5 65 353.0 66 289.0 67 248.0 68 223.0 69 186.5 70 151.5 71 128.0 72 108.5 73 86.5 74 73.0 75 62.0 76 55.5 77 45.0 78 35.0 79 29.0 80 24.0 81 20.5 82 16.5 83 11.0 84 12.0 85 13.5 86 11.5 87 9.0 88 7.5 89 5.5 90 5.5 91 5.5 92 5.0 93 4.5 94 5.0 95 7.0 96 8.0 97 10.0 98 52.5 99 1364.5 100 2636.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022496766089874578 2 0.0018747305074895482 3 0.0 4 0.0 5 0.0 6 0.0018747305074895482 7 0.0028120957612343222 8 0.0 9 0.0 10-11 0.0 12-13 0.0056241915224686445 14-15 0.0 16-17 0.004218143641851484 18-19 4.6868262687238706E-4 20-21 0.0037494610149790965 22-23 4.6868262687238706E-4 24-25 0.0023434131343619356 26-27 0.004686826268723871 28-29 0.004686826268723871 30-31 9.373652537447741E-4 32-33 0.0023434131343619356 34-35 0.0 36-37 0.0 38-39 0.0018747305074895482 40-41 0.004218143641851484 42-43 0.00328077838810671 44-45 0.0023434131343619356 46-47 0.0028120957612343222 48-49 0.0 50-51 0.0023434131343619356 52-53 0.0028120957612343222 54-55 0.004218143641851484 56-57 0.012654430925554452 58-59 0.0014060478806171611 60-61 0.0018747305074895482 62-63 0.0 64-65 0.010779700418064904 66-67 0.0023434131343619356 68-69 0.0018747305074895482 70-71 0.0060928741493410325 72-73 4.6868262687238706E-4 74-75 0.007030239403085807 76 0.0056241915224686445 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 106682.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.804709323034814 #Duplication Level Percentage of deduplicated Percentage of total 1 69.49623064384679 25.577885678933654 2 9.79777913610432 7.212088262312292 3 6.5785452322738385 7.2636433512682546 4 4.821210268948655 7.097729701355431 5 3.099531377343113 5.703867569036951 6 1.8133659331703342 4.004424363997675 7 1.2530562347188265 3.2282859338970025 8 0.8149959250203749 2.399655049586622 9 0.496638141809291 1.6450760203220787 >10 1.7344132029339852 9.419583434881236 >50 0.061124694376528114 1.6216418889784594 >100 0.017828035859820703 1.6244539847396937 >500 0.0025468622656886716 0.610224780187848 >1k 0.010187449062754686 7.985414596651731 >5k 0.0 0.0 >10k+ 0.0025468622656886716 14.60602538385107 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGT 15582 14.60602538385107 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCG 2754 2.581503908813108 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 2672 2.5046399580060363 No Hit ATTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGT 1652 1.548527399186367 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCC 1441 1.3507433306462198 No Hit CTTAAACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGT 651 0.610224780187848 No Hit ATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGTCTT 494 0.4630584353499185 Illumina PCR Primer Index 4 (95% over 22bp) TCTTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCC 355 0.33276466507939484 No Hit CTTATACACATCTCCGCGCCCACGAGACTTCTACCAATCTCGTATGCCGT 297 0.27839748036219797 No Hit CTTATACACATCTCCGAGCCCACGAGACTTCTACCACTCTCGTATGCCGT 183 0.1715378414352937 No Hit CTTATACACATCTCCGAGCCCACGAGACTTCTACCAATATCGTATGCCGT 175 0.1640389194053355 No Hit CTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGTC 118 0.11060909994188337 No Hit CTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGACGT 111 0.10404754316566994 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.032807783881067094 0.0 2 0.0 0.0 0.0 0.032807783881067094 0.0 3 0.0 0.0 0.0 0.032807783881067094 0.0 4 0.0 0.0 0.0 0.03374514913481187 0.0 5 0.0 0.0 0.0 0.041244071164770066 0.0 6 0.0 0.0 0.0 0.041244071164770066 0.0 7 0.0 0.0 0.0 0.04686826268723871 0.0 8 0.0 0.0 0.0 0.04686826268723871 0.0 9 0.0 0.0 0.0 0.05624191522468645 0.0 10 0.0 0.0 0.0 0.05905401098592077 0.0 11 0.0 0.0 0.0 0.05905401098592077 0.0 12 0.0 0.0 0.0 0.06374083725464465 0.0 13 0.0 0.0 0.0 0.06374083725464465 0.0 14 0.0 0.0 0.0 0.06467820250838942 0.0 15 0.0 0.0 0.0 0.06749029826962374 0.0 16 0.0 0.0 0.0 0.06749029826962374 0.0 17 0.0 0.0 0.0 0.06749029826962374 0.0 18 0.0 0.0 0.0 0.06749029826962374 0.0 19 0.0 0.0 0.0 0.06936502877711329 0.0 20 0.0 0.0 0.0 0.07217712453834761 0.0 21 0.0 0.0 0.0 0.07217712453834761 0.0 22 0.0 0.0 0.0 0.0796760465683058 0.0 23 0.0 0.0 0.0 0.08623760334451923 0.0 24 0.0 0.0 0.0 0.08811233385200877 0.0 25 0.0 0.0 0.0 0.08904969910575355 0.0 26 0.0 0.0 0.0 0.09279916012073264 0.0 27 0.0 0.0 0.0 0.10311017791192516 0.0 28 0.0 0.0 0.0 0.14060478806171614 0.0 29 0.0 0.0 0.0 0.1780993982115071 0.0 30 0.0 0.0 0.0 0.2071577210775951 0.0 31 0.0 0.0 0.0 0.25683807952606813 0.0 32 0.0 0.0 0.0 0.32432837779569185 0.0 33 0.0 0.0 0.0 0.3955681370802947 0.0 34 0.0 0.0 0.0 0.48461783618604826 0.0 35 0.0 0.0 0.0 0.6186610674715509 0.0 36 0.0 0.0 0.0 0.8267561538028908 0.0 37 0.0 0.0 0.0 1.2298232129131437 0.0 38 0.0 0.0 0.0 1.6722596126806772 0.0 39 0.0 0.0 0.0 2.166251101404173 0.0 40 0.0 0.0 0.0 2.8083463002193434 0.0 41 0.0 0.0 0.0 3.409197427869744 0.0 42 0.0 0.0 0.0 4.073789392774788 0.0 43 0.0 0.0 0.0 4.7711891415609005 0.0 44 0.0 0.0 0.0 5.474213081869482 0.0 45 0.0 0.0 0.0 6.175362291670572 0.0 46 0.0 0.0 0.0 6.878386231979153 0.0 47 0.0 0.0 0.0 7.558913406197859 0.0 48 0.0 0.0 0.0 8.153202977072047 0.0 49 0.0 0.0 0.0 8.846853264843178 0.0 50 0.0 0.0 0.0 9.460827506046005 0.0 51 0.0 0.0 0.0 10.076676477756322 0.0 52 0.0 0.0 0.0 10.69252544946664 0.0 53 0.0 0.0 0.0 11.241821488161078 0.0 54 0.0 0.0 0.0 11.791117526855514 0.0 55 0.0 0.0 0.0 12.313229973191353 0.0 56 0.0 0.0 0.0 12.80909619242234 0.0 57 0.0 0.0 0.0 13.359329596370522 0.0 58 0.0 0.0 0.0 13.8655068333927 0.0 59 0.0 0.0 0.0 14.366997244146154 0.0 60 0.0 0.0 0.0 14.887234959974503 0.0 61 0.0 0.0 0.0 15.353105491085657 0.0 62 0.0 0.0 0.0 15.742112071389737 0.0 63 0.0 0.0 0.0 16.139554938977522 0.0 64 0.0 0.0 0.0 16.604488104834928 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGGATC 15 0.0022164683 70.02299 11 GGGTATC 15 0.0022164683 70.02299 9 ATCATGG 15 0.0022164683 70.02299 8 AACGTAT 15 0.0022205971 69.99016 39 TAACGTA 15 0.0022205971 69.99016 38 CCAAGCG 15 0.0022205971 69.99016 34 CCCGAGA 25 2.8257073E-6 69.99016 21 AGCCGTC 60 0.0 64.15764 45 AAGCCGT 60 0.0 64.15764 44 AGGGGGG 2235 0.0 63.88363 70 AAACTCG 30 8.335241E-6 58.32513 36 TCTGCTA 105 0.0 56.685276 53 CTTAAAC 210 0.0 54.992268 1 CCGTCTA 70 0.0 54.992264 47 CTGATTG 45 2.665729E-8 54.46232 54 AATATCG 90 0.0 54.436787 36 ATATCGT 90 0.0 54.436787 37 CGTATGA 105 0.0 53.325836 41 CATGGAA 20 0.006925837 52.51724 10 TGGAATA 20 0.006925837 52.51724 12 >>END_MODULE