FastQCFastQC Report
Wed 25 May 2016
SRR1780867_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780867_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214017
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2630612.29154693318755No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG12840.5999523402346543No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCAT150.002223130369.994399
CGTCATC200.006946547452.49579210
TCTATCG200.006946547452.49579231
GTTGTAA305.8641814E-446.6738361
TCGGTGG1550.045.1576745
CTCGGTG1500.044.3401444
CGGTGGT1600.043.74649446
GCCGTAT1450.041.04078754
ATGGGCG350.001253974339.9967969
GAGGTTC350.001253974339.99679668
TGGTCGC1600.039.37184549
TCTCGGT1700.039.12365343
GATCTCG1700.039.12365341
GTAGATC1800.038.8948638
TTGCGAT459.168514E-538.8948616
TCGCCGT1650.038.18768352
TGTAGGT556.677372E-638.17875737
GTCGCCG1750.037.99695651
ATCTCGG1750.036.00552742
GTTAGTG501.7043043E-435.005383
Produced by FastQC (version 0.11.5)