FastQCFastQC Report
Wed 25 May 2016
SRR1780867_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780867_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214017
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT135796.344823074802469No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23351.0910348243363845No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCG23351.0910348243363845No Hit
ATTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT18620.8700243438605345No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCC15270.7134947223818668No Hit
CTTAAACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT5730.2677357406187359No Hit
ATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGTCTT4880.2280192694972829RNA PCR Primer, Index 15 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCC3270.1527916006672367No Hit
CTTATACACATCTCCGCGCCCACGAGACGCTTGCCAATCTCGTATGCCGT2680.12522369718293405No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA207.9022575E-570.02080548
ACGATTG252.8320246E-670.0044427
AGGGGGG21200.063.5802170
AACTCGT352.967954E-759.98978437
ATCGTAT800.056.8653239
TTCGGCA407.4522177E-752.5033325
TGCCGAC407.4522177E-752.5033345
AAACTCG407.4642594E-752.49106636
AGCGTCA200.006949023352.4910663
GCCGTCT27050.052.01808546
CGTCTTC27100.051.9342548
CCGTCTT26900.051.9177947
TCGTATG28150.051.8384240
CGTATGC27750.051.7029141
TATGCCG27400.051.59705743
ATATCGT950.051.57016837
TGCCGTC27300.051.41351745
ATCTCGT27400.051.34162537
CTCGTAT27550.051.31612839
AATCTCG27650.051.25709536