FastQCFastQC Report
Wed 25 May 2016
SRR1780866_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780866_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191072
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2890515.1278052252554No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG14970.783474292413331No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCCG150.002218631870.0272338
CTATCGG200.0069397252.50667627
GCGAAGT200.0069397252.50667632
CCATATT200.0069397252.50667632
TCCGTGC200.0069397252.50667636
TTCGGTA200.0069397252.50667658
TGCCGTA200.0069397252.50667640
GTGCCGT200.0069397252.50667639
GAATAAC200.006946909252.4929365
CTTTGCG200.006946909252.4929324
GCCGTAT2750.043.27823354
TCGCCGT2550.041.181752
CGCCGTA2750.040.73245253
CAACAGT350.00125235940.00508536
CCGTATC2800.040.00508555
GCGCTGG350.00125235940.00508515
CTCTAAC350.00125235940.0050851
GTAGATC2900.039.8430838
CGTATCA3000.039.6717156
GTCGCCG2650.039.6173151
Produced by FastQC (version 0.11.5)