##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780866_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 191072 Sequences flagged as poor quality 0 Sequence length 76 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.4980635571931 32.0 27.0 32.0 22.0 32.0 2 29.28306083570591 32.0 32.0 32.0 22.0 32.0 3 29.13370875900184 32.0 32.0 32.0 22.0 32.0 4 28.46018254898677 32.0 27.0 32.0 13.0 32.0 5 28.96276796181544 32.0 32.0 32.0 13.0 32.0 6 32.95566592698041 37.0 32.0 37.0 13.0 37.0 7 32.68410337464411 37.0 32.0 37.0 13.0 37.0 8 32.85041764361078 37.0 32.0 37.0 13.0 37.0 9 32.921061170658184 37.0 32.0 37.0 13.0 37.0 10-11 32.70364836292078 37.0 32.0 37.0 13.0 37.0 12-13 32.5563583361246 37.0 32.0 37.0 13.0 37.0 14-15 33.219788875397754 37.0 34.5 37.0 17.5 37.0 16-17 33.232849397085914 37.0 34.5 37.0 17.5 37.0 18-19 32.683972533913916 37.0 32.0 37.0 13.0 37.0 20-21 33.11886356975381 37.0 34.5 37.0 17.5 37.0 22-23 33.048413686987104 37.0 34.5 37.0 17.5 37.0 24-25 32.60357613883772 37.0 32.0 37.0 13.0 37.0 26-27 33.01584481242673 37.0 34.5 37.0 17.5 37.0 28-29 32.87615139842572 37.0 32.0 37.0 13.0 37.0 30-31 32.68418449589683 37.0 32.0 37.0 13.0 37.0 32-33 32.843137665382685 37.0 32.0 37.0 13.0 37.0 34-35 32.66673034248869 37.0 32.0 37.0 13.0 37.0 36-37 33.26364145453023 37.0 34.5 37.0 17.5 37.0 38-39 33.301378537933346 37.0 34.5 37.0 17.5 37.0 40-41 32.815025226092786 37.0 34.5 37.0 17.5 37.0 42-43 32.661420825657345 37.0 32.0 37.0 13.0 37.0 44-45 32.35217875983922 37.0 32.0 37.0 13.0 37.0 46-47 32.64049939289901 37.0 34.5 37.0 13.0 37.0 48-49 33.11500376821303 37.0 34.5 37.0 17.5 37.0 50-51 33.356855007536424 37.0 37.0 37.0 22.0 37.0 52-53 32.41103353709596 37.0 32.0 37.0 13.0 37.0 54-55 32.50840520850779 37.0 32.0 37.0 13.0 37.0 56-57 32.30245143192095 37.0 32.0 37.0 13.0 37.0 58-59 31.980072956791158 37.0 32.0 37.0 13.0 37.0 60-61 31.73981274074694 37.0 32.0 37.0 13.0 37.0 62-63 31.473708863674425 37.0 29.5 37.0 13.0 37.0 64-65 31.446357917434263 37.0 29.5 37.0 13.0 37.0 66-67 31.459711522358063 37.0 29.5 37.0 13.0 37.0 68-69 31.65964924217049 37.0 32.0 37.0 13.0 37.0 70-71 31.750630652319543 37.0 32.0 37.0 13.0 37.0 72-73 32.011495666555014 37.0 32.0 37.0 13.0 37.0 74-75 32.2330378077374 37.0 32.0 37.0 13.0 37.0 76 31.95935040194272 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 -0.43575048786752646 11101 2 0.03525903689742549 11101 3 0.38122652585175487 11101 4 0.6426550742944777 11101 5 -0.12583583269858067 11101 6 -0.5000828772641555 11101 7 -0.8574181293129257 11101 8 1.178603419240467 11101 9 -0.07213849497071578 11101 10-11 -0.4800576272575583 11101 12-13 0.6957162045239471 11101 14-15 -0.4190200062659599 11101 16-17 -0.02427603423713265 11101 18-19 0.5129720869796515 11101 20-21 -0.535104399469823 11101 22-23 0.12686894897418455 11101 24-25 -0.36095148200966776 11101 26-27 0.27107743066802925 11101 28-29 0.20178565078197153 11101 30-31 -0.47737837625648893 11101 32-33 -0.0841167868112862 11101 34-35 0.18763169907511212 11101 36-37 -0.9244364037090733 11101 38-39 0.16875293105467648 11101 40-41 -0.1526635266577756 11101 42-43 -0.39192435222489763 11101 44-45 -0.5852466956491753 11101 46-47 -0.6619714964155143 11101 48-49 0.3640004051143322 11101 50-51 -0.7899283124385263 11101 52-53 -0.0801211189425004 11101 54-55 -0.047528487535259956 11101 56-57 0.5091735430130839 11101 58-59 -0.2740652336438849 11101 60-61 0.2509855779462562 11101 62-63 0.4099747676640746 11101 64-65 0.47614023519535564 11101 66-67 -0.9875803725991616 11101 68-69 0.221755167978948 11101 70-71 -0.6773720565784203 11101 72-73 -0.28994239221730567 11101 74-75 0.2359489661385652 11101 76 -1.3013041394685416 11102 1 0.32467934778354746 11102 2 0.6786692770997007 11102 3 0.04051856124998565 11102 4 0.5623769453437824 11102 5 -0.018376919929934843 11102 6 0.7257388673628995 11102 7 1.2407171424949155 11102 8 0.7181103724642384 11102 9 0.7527666883415449 11102 10-11 0.2567075434124817 11102 12-13 1.1716564700106744 11102 14-15 0.0207318900677933 11102 16-17 1.1599448886452919 11102 18-19 0.5824648177002558 11102 20-21 0.5970463590636825 11102 22-23 0.6563411360791136 11102 24-25 0.8228348643872891 11102 26-27 0.4630812233355357 11102 28-29 0.6299620098211633 11102 30-31 0.6450141648307408 11102 32-33 0.4537308743770865 11102 34-35 0.5165270846629682 11102 36-37 0.24528546734023138 11102 38-39 0.5843660789687135 11102 40-41 0.32861017751415744 11102 42-43 -0.4695160083563721 11102 44-45 0.09419388589317634 11102 46-47 0.1880601091470595 11102 48-49 0.6294871307780525 11102 50-51 0.03667566986235471 11102 52-53 0.2485767318792398 11102 54-55 0.27149015974666924 11102 56-57 0.6875079298651698 11102 58-59 -0.006129076880295514 11102 60-61 0.6972561215619386 11102 62-63 1.1457522645035212 11102 64-65 1.5426699444241763 11102 66-67 0.5086269598913553 11102 68-69 0.5593815902292647 11102 70-71 0.5782379307793519 11102 72-73 1.3281043840153188 11102 74-75 1.1171910647479137 11102 76 -0.8826884631095027 11103 1 0.24705822883949224 11103 2 0.24061617975456784 11103 3 0.5422434750042981 11103 4 -0.5982650225578254 11103 5 -1.118571910180421 11103 6 -0.3635816666104006 11103 7 -0.31050529638314117 11103 8 0.17678743861092983 11103 9 -0.06124261119347807 11103 10-11 -0.8527119856110659 11103 12-13 -0.9255761683574093 11103 14-15 -0.9389201273313361 11103 16-17 0.4706543531720726 11103 18-19 -0.21062052803245734 11103 20-21 -0.37749241399766476 11103 22-23 0.12671761725505348 11103 24-25 -0.38122993237286806 11103 26-27 0.9208958326050762 11103 28-29 0.17045998492240244 11103 30-31 -0.1978686910264571 11103 32-33 -0.9005514115086228 11103 34-35 0.7595899315206296 11103 36-37 0.2329485841843919 11103 38-39 -0.5322155919477467 11103 40-41 -0.573819700991919 11103 42-43 -0.46486624084474926 11103 44-45 -0.24913894746515552 11103 46-47 -0.09992549157290398 11103 48-49 -0.7631182389534601 11103 50-51 -0.6421528887097097 11103 52-53 -0.6915012642209533 11103 54-55 -0.18486202264421792 11103 56-57 -0.4492102342266264 11103 58-59 -0.7557540956293565 11103 60-61 -1.1297650273806177 11103 62-63 -1.0022528352414923 11103 64-65 -0.7729517744898757 11103 66-67 -0.7339484113400836 11103 68-69 -0.5374760668878764 11103 70-71 -0.7892061970142592 11103 72-73 -1.772690576236677 11103 74-75 -0.4421684672754793 11103 76 -1.2773937278462668 11104 1 -1.0607504878675265 11104 2 -1.0450981059597169 11104 3 0.3722979544231819 11104 4 -0.19663063999123764 11104 5 -0.8222644041271536 11104 6 -1.625082877264152 11104 7 -0.2770609864557798 11104 8 0.2768177049547518 11104 9 -0.2596384949707158 11104 10-11 0.04226380131387231 11104 12-13 -1.0766052240474835 11104 14-15 -0.4190200062659599 11104 16-17 0.41768825147715205 11104 18-19 0.5486863726939362 11104 20-21 -0.316354399469823 11104 22-23 -0.7347381938829614 11104 24-25 -0.12434433915252896 11104 26-27 -0.0012439979034013504 11104 28-29 0.14821422221054092 11104 30-31 -0.7363069476850583 11104 32-33 -0.5841167868112862 11104 34-35 0.16084598478939682 11104 36-37 -0.24140068942335802 11104 38-39 -0.06338992608817762 11104 40-41 -0.161592098086345 11104 42-43 -1.2401386379391788 11104 44-45 -0.2727466956491753 11104 46-47 -0.08161435355837199 11104 48-49 -0.5958210234570984 11104 50-51 -0.31224974100995695 11104 52-53 -0.10690683322822281 11104 54-55 0.31407865532188595 11104 56-57 0.08506640015593803 11104 58-59 -1.0285295193581696 11104 60-61 -0.6195501363394591 11104 62-63 0.44122476766407814 11104 64-65 0.306497378052498 11104 66-67 0.43206248454369245 11104 68-69 -1.6800305463067673 11104 70-71 -0.33362205657842026 11104 72-73 -0.017620963645875065 11104 74-75 -0.5988724624328654 11104 76 0.046910146245739526 11105 1 0.5106780835610429 11105 2 -0.13438382024543216 11105 3 0.006226525851754872 11105 4 1.026583645723047 11105 5 -0.456192975555723 11105 6 -0.41079716297844016 11105 7 -0.3306324150272104 11105 8 -0.678539437902387 11105 9 0.15107579074356892 11105 10-11 -0.10505762725755119 11105 12-13 -0.11678379547605289 11105 14-15 -0.2940200062659599 11105 16-17 -0.44838317709427855 11105 18-19 0.12457922983679026 11105 20-21 -0.3029615423269618 11105 22-23 -0.9490239081686767 11105 24-25 0.009584232276040439 11105 26-27 -0.22445828361768605 11105 28-29 0.06339279363911032 11105 30-31 -0.14702123339934303 11105 32-33 0.7105260703315679 11105 34-35 -0.23647544378203378 11105 36-37 1.0041350248623573 11105 38-39 -0.3624970689453235 11105 40-41 0.2893007590565091 11105 42-43 -0.052638637939175226 11105 44-45 0.6647533043508247 11105 46-47 0.10588564644162801 11105 48-49 1.1452504051143322 11105 50-51 0.5225716875614737 11105 52-53 -0.0979782617996463 11105 54-55 -0.8198499161066835 11105 56-57 0.09399497158451453 11105 58-59 -0.6222795193581696 11105 60-61 0.5500927208034021 11105 62-63 -0.8400252323359254 11105 64-65 -1.1577883362332173 11105 66-67 0.1909910559722654 11105 68-69 0.7485408822646633 11105 70-71 -0.22647919943556616 11105 72-73 -0.9461923922173057 11105 74-75 0.03952039470998869 11105 76 -0.4263041394685416 11106 1 0.2667339841821601 11106 2 0.3058335710589155 11106 3 -0.43181695240911466 11106 4 -0.2113821927862709 11106 5 0.3735430492889975 11106 6 0.6264699177669044 11106 7 0.36230236758148493 11106 8 0.04723695961313723 11106 9 -0.63176582416326 11106 10-11 -0.07776725458674605 11106 12-13 -0.5360772737369217 11106 14-15 -0.37922963359515904 11106 16-17 -1.2203552267837168 11106 18-19 -0.4995667328961275 11106 20-21 -0.6455469460536705 11106 22-23 -1.299295647299111 11106 24-25 0.09821000867356133 11106 26-27 -0.402059215294706 11106 28-29 -0.4099379517025028 11106 30-31 -0.3731081899210835 11106 32-33 -0.5638528116560053 11106 34-35 -0.6211027729745808 11106 36-37 -0.7545606273115553 11106 38-39 -0.9060917894422147 11106 40-41 0.7834389578142691 11106 42-43 -1.0206510602994285 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0.9486452417824935 11108 6 0.8297400775588031 11108 7 -1.0843717312665255 11108 8 -1.2075382169011668 11108 9 -0.22537414820637025 11108 10-11 0.36842375247382364 11108 12-13 0.6094448370025844 11108 14-15 0.5763630828671324 11108 16-17 -0.1645767057878018 11108 18-19 0.408385701143267 11108 20-21 -0.26018834330376706 11108 22-23 -0.6453380107327789 11108 24-25 -0.13155587761406906 11108 26-27 0.03588207047266678 11108 28-29 -0.18351654702023268 11108 30-31 0.23332064694253773 11108 32-33 0.9618996967052027 11108 34-35 -0.2753948577014427 11108 36-37 -0.25013847941114165 11108 38-39 0.026201038502783547 11108 40-41 0.6500684635742147 11108 42-43 0.33594500064446464 11108 44-45 0.7556415827391092 11108 46-47 0.29330933386531655 11108 48-49 -0.4778799367660085 11108 50-51 -0.49906292782313955 11108 52-53 0.0627360239146384 11108 54-55 -0.04115638741316019 11108 56-57 -0.2792116828721447 11108 58-59 0.11810509602644359 11108 60-61 0.9060527330134143 11108 62-63 -0.08819373050442181 11108 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-0.01621134450641648 21611 54-55 -0.40443638227210243 21611 56-57 -0.425506908114734 21611 58-59 0.6906904054538607 21611 60-61 0.11188783358535304 21611 62-63 -0.12761921729832792 21611 64-65 -0.36314547909035966 21611 66-67 -0.3860766132006681 21611 68-69 0.6103829875278208 21611 70-71 0.02234598853436154 21611 72-73 0.2824260288353315 21611 74-75 1.1020203947099958 21611 76 -0.7195372221753118 21612 1 0.3921015162434678 21612 2 0.7406161797545678 21612 3 1.4207948711754952 21612 4 0.5208667906356901 21612 5 0.44148174181323796 21612 6 1.5285656324994648 21612 7 0.6275510382101999 21612 8 0.4882128745333745 21612 9 0.5018594495308264 21612 10-11 0.26001816924809873 21612 12-13 0.7035849095599218 21612 14-15 0.3664694593044402 21612 16-17 0.5300983234196011 21612 18-19 0.5419096512139774 21612 20-21 0.29361476805330256 21612 22-23 0.7449958760039905 21612 24-25 0.14071861048775958 21612 26-27 0.13297362799588086 21612 28-29 1.0641314883975923 21612 30-31 1.4249212126438238 21612 32-33 0.6423092152442109 21612 34-35 0.921155594244695 21612 36-37 0.21456667953861341 21612 38-39 0.298506271239674 21612 40-41 -0.08168459551697538 21612 42-43 0.074031454558245 21612 44-45 -0.5098998302843256 21612 46-47 -0.7882561829519972 21612 48-49 0.29925220367548633 21612 50-51 0.9328684501513962 21612 52-53 0.264174872835504 21612 54-55 0.41524772007008437 21612 56-57 0.2616786817592285 21612 58-59 -0.31382628194809925 21612 60-61 0.370204488532071 21612 62-63 0.39815565152841614 21612 64-65 -0.11751341331440557 21612 66-67 0.3248553930740137 21612 68-69 0.1972818894589068 21612 70-71 0.8288586731235341 21612 72-73 0.8586958400540254 21612 74-75 0.1724212169093704 21612 76 0.6569435480956933 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 3.0 11 13.0 12 40.0 13 109.0 14 177.0 15 286.0 16 409.0 17 596.0 18 792.0 19 1004.0 20 1258.0 21 1823.0 22 2438.0 23 3209.0 24 3815.0 25 4486.0 26 4866.0 27 5689.0 28 6211.0 29 7431.0 30 8824.0 31 10760.0 32 13734.0 33 19080.0 34 28458.0 35 39653.0 36 25907.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 56.94613818478722 14.558548750935554 6.986700721751464 21.508612342525762 2 37.69718730045744 20.725209617820394 22.883147878699063 18.694455203023104 3 34.39049029819887 19.861604736007372 24.808031531507954 20.939873434285804 4 30.029465795079265 17.376968529693464 27.666445106218173 24.9271205690091 5 28.99124918877049 26.35972533338916 29.628718571398664 15.020306906441686 6 39.957816286263075 30.52091129382579 16.851503906965544 12.669768512945584 7 40.61068862103076 26.28138362738992 16.724326531039498 16.383601220539823 8 38.725159237335596 28.694646380589006 16.54498160331193 16.03521277876347 9 37.485999602231686 18.417196152114975 16.065652706394648 28.031151539258687 10-11 37.8467070193206 21.932792681043935 21.90165152787918 18.318848771756283 12-13 37.81739769869495 21.425898448437838 21.011642604237814 19.745061248629398 14-15 37.52574459788705 21.287347658713646 19.21940547316412 21.96750227023519 16-17 37.00996556019638 23.666896963225827 19.69742172533995 19.625715751237845 18-19 35.57758487583646 24.118005741696916 21.56616952399396 18.738239858472667 20-21 36.47866388221563 23.691124824009087 21.111320468332103 18.718890825443182 22-23 36.69265251968566 23.59452648437316 20.79888833933913 18.913932656602057 24-25 36.135630300920894 24.361534254318588 20.52118798200598 18.98164746275453 26-27 36.33775321416141 23.986685403402443 21.442571643154157 18.232989739281987 28-29 36.631505125071634 23.85794772086743 21.514310163002662 17.99623699105827 30-31 36.65329470904871 23.958832164922555 21.39042191012265 17.99745121590609 32-33 36.278660811949486 24.18865238576236 21.502216453569613 18.030470348718538 34-35 35.94376826916283 23.93145841892744 22.080793859729468 18.043979452180263 36-37 36.95175438596491 22.719873968931875 22.4801637147762 17.84820793032701 38-39 36.61307290112892 22.90426918306144 23.015486714084588 17.467171201725048 40-41 36.578702201379656 22.615433733552457 22.742094189320746 18.06376987574714 42-43 35.80308941940583 22.57976725990009 23.15128106494166 18.465862255752427 44-45 35.11921429263046 21.968089143138275 23.44456162480013 19.46813493943113 46-47 35.708154506437765 21.318433999790642 22.97367319166754 19.99973830210405 48-49 35.18586849852797 21.539810271508014 24.705789990186457 18.56853123977756 50-51 35.46483480536474 21.467320902845927 23.86915276414786 19.19869152764148 52-53 34.0865792255917 22.084454260920538 24.055542180025334 19.773424333462437 54-55 34.09545891090275 22.58297503748695 22.951433139770707 20.370132911839594 56-57 33.88690582114706 22.542420784876114 23.110037579424482 20.460635814552344 58-59 33.68725742416193 22.589290154070827 23.760982158980575 19.962470262786674 60-61 33.639158935978124 22.693550708519243 23.190495505515067 20.47679484998757 62-63 33.81136065067543 22.99058425754856 23.189994923140535 20.008060168635478 64-65 34.487848153604986 22.41294033596512 22.893937292442484 20.2052742179874 66-67 34.73203233564589 23.14343512589076 21.240091385622726 20.884441152840623 68-69 34.829737566602816 23.188769902333323 20.83346418365104 21.14802834741283 70-71 35.580161681614406 23.041854549356337 20.635473124160907 20.742510644868347 72-73 36.40122471410253 22.922565619030173 20.094208777117732 20.582000889749562 74-75 37.282409588109076 23.1336682891087 19.617679384518762 19.966242738263464 76 38.75949923587414 22.43232775765696 19.440722674649862 19.36745033181904 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 2.0 8 4.0 9 5.5 10 7.0 11 10.0 12 13.0 13 15.0 14 19.0 15 29.5 16 53.0 17 68.0 18 79.0 19 119.0 20 184.5 21 221.0 22 280.0 23 390.5 24 516.5 25 591.0 26 706.5 27 1024.5 28 1274.0 29 1321.0 30 1585.0 31 2159.5 32 2623.5 33 2777.0 34 3200.0 35 4032.0 36 4777.0 37 5113.0 38 5658.5 39 6738.0 40 7931.5 41 9010.5 42 9430.0 43 9906.5 44 10766.5 45 10642.0 46 10134.0 47 9891.5 48 9377.5 49 8689.5 50 8273.0 51 7739.5 52 6566.0 53 5429.0 54 4932.0 55 4623.5 56 3887.5 57 3029.5 58 2599.0 59 2340.5 60 1836.5 61 1412.5 62 1234.0 63 1143.5 64 961.0 65 821.5 66 688.5 67 603.0 68 601.0 69 577.0 70 483.5 71 412.0 72 420.0 73 414.0 74 382.0 75 364.0 76 334.5 77 298.0 78 272.0 79 253.0 80 246.5 81 219.5 82 183.5 83 168.0 84 176.0 85 179.5 86 163.5 87 152.0 88 170.5 89 197.5 90 211.0 91 233.5 92 251.0 93 264.0 94 328.5 95 451.5 96 523.0 97 667.0 98 1164.0 99 15504.5 100 29492.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004710266287054094 2 0.005233629207837883 3 0.013084073019594708 4 0.001570088762351365 5 0.002093451683135153 6 0.001570088762351365 7 0.004710266287054094 8 0.0026168146039189416 9 0.00314017752470273 10-11 0.003401858985094624 12-13 0.003401858985094624 14-15 0.006542036509797354 16-17 0.0073270808909730355 18-19 0.007065399430581142 20-21 0.005756992128621671 22-23 0.003401858985094624 24-25 0.004448584826662201 26-27 0.0018317702227432589 28-29 7.850443811756825E-4 30-31 2.6168146039189414E-4 32-33 5.233629207837883E-4 34-35 0.0028784960643108357 36-37 0.004186903366270306 38-39 0.0026168146039189416 40-41 0.005233629207837883 42-43 2.6168146039189414E-4 44-45 0.004448584826662201 46-47 0.00628035504940546 48-49 0.004971947747445989 50-51 0.004971947747445989 52-53 0.005233629207837883 54-55 0.0028784960643108357 56-57 0.005233629207837883 58-59 0.012299028638419024 60-61 0.002355133143527047 62-63 0.004710266287054094 64-65 0.005495310668229778 66-67 0.007065399430581142 68-69 0.0073270808909730355 70-71 0.009158851113716296 72-73 0.0036635404454865178 74-75 0.0010467258415675766 76 0.002093451683135153 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 191072.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.4165340813934 #Duplication Level Percentage of deduplicated Percentage of total 1 73.43864515328846 34.0876737564897 2 8.656090383249332 8.035714285714286 3 6.3536627992197445 8.847450175849941 4 4.302675641849609 7.988611622843744 5 2.7297635557960964 6.335308156087757 6 1.497367204501122 4.1701557528052255 7 0.933599431722085 3.033411488862837 8 0.5694054505068272 2.114386199966505 9 0.3337505214851898 1.394238820968012 >10 1.1737645029259547 7.69919192765031 >50 0.006765213273348442 0.2145787975213532 >100 0.0022550710911161476 0.16799949757159605 >500 0.0 0.0 >1k 0.0011275355455580738 0.783474292413331 >5k 0.0 0.0 >10k+ 0.0011275355455580738 15.1278052252554 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 28905 15.1278052252554 No Hit CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG 1497 0.783474292413331 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004710266287054095 0.0 2 0.0 0.0 0.0 0.004710266287054095 0.0 3 0.0 0.0 0.0 0.004710266287054095 0.0 4 0.0 0.0 0.0 0.006803717970189248 0.0 5 0.0 0.0 0.0 0.008373806732540613 0.0 6 0.0 0.0 0.0 0.008373806732540613 0.0 7 0.0 0.0 0.0 0.008373806732540613 0.0 8 0.0 0.0 0.0 0.008897169653324401 0.0 9 0.0 0.0 0.0 0.011513984257243343 0.0 10 0.0 0.0 0.0 0.014130798861162284 0.0 11 0.0 0.0 0.0 0.017270976385865014 0.0 12 0.0 0.0 0.0 0.01884106514821638 0.0 13 0.0 0.0 0.0 0.019887790989783955 0.0 14 0.0 0.0 0.0 0.022504605593702896 0.0 15 0.0 0.0 0.0 0.023551331435270474 0.0 16 0.0 0.0 0.0 0.024074694356054263 0.0 17 0.0 0.0 0.0 0.025644783118405626 0.0 18 0.0 0.0 0.0 0.027738234801540782 0.0 19 0.0 0.0 0.0 0.03401858985094624 0.0 20 0.0 0.0 0.0 0.036635404454865184 0.0 21 0.0 0.0 0.0 0.03768213029643276 0.0 22 0.0 0.0 0.0 0.04762602579132474 0.0 23 0.0 0.0 0.0 0.049196114553676104 0.0 24 0.0 0.0 0.0 0.0580932842070005 0.0 25 0.0 0.0 0.0 0.05966337296935187 0.0 26 0.0 0.0 0.0 0.06437363925640596 0.0 27 0.0 0.0 0.0 0.08478479316697371 0.0 28 0.0 0.0 0.0 0.12403701222575783 0.0 29 0.0 0.0 0.0 0.15386869871043377 0.0 30 0.0 0.0 0.0 0.20777507955116395 0.0 31 0.0 0.0 0.0 0.27162535588678616 0.0 32 0.0 0.0 0.0 0.3438494389549489 0.0 33 0.0 0.0 0.0 0.43177440964662533 0.0 34 0.0 0.0 0.0 0.5280731870708424 0.0 35 0.0 0.0 0.0 0.6714746273656004 0.0 36 0.0 0.0 0.0 0.8766328923128455 0.0 37 0.0 0.0 0.0 1.2267626863171999 0.0 38 0.0 0.0 0.0 1.6998827667057443 0.0 39 0.0 0.0 0.0 2.218535421202479 0.0 40 0.0 0.0 0.0 2.8664587171328084 0.0 41 0.0 0.0 0.0 3.602306983754815 0.0 42 0.0 0.0 0.0 4.493594037849607 0.0 43 0.0 0.0 0.0 5.305853290906046 0.0 44 0.0 0.0 0.0 6.183009546139675 0.0 45 0.0 0.0 0.0 7.047605091274494 0.0 46 0.0 0.0 0.0 8.032574108189584 0.0 47 0.0 0.0 0.0 8.96887037347178 0.0 48 0.0 0.0 0.0 9.822475297270138 0.0 49 0.0 0.0 0.0 10.586061798693686 0.0 50 0.0 0.0 0.0 11.291555015910232 0.0 51 0.0 0.0 0.0 12.115328253223916 0.0 52 0.0 0.0 0.0 12.899849271478814 0.0 53 0.0 0.0 0.0 13.724145871713281 0.0 54 0.0 0.0 0.0 14.552106012393233 0.0 55 0.0 0.0 0.0 15.386346508122593 0.0 56 0.0 0.0 0.0 16.103877072517168 0.0 57 0.0 0.0 0.0 16.884734550326577 0.0 58 0.0 0.0 0.0 17.55359236308826 0.0 59 0.0 0.0 0.0 18.159646625355887 0.0 60 0.0 0.0 0.0 18.701327248367107 0.0 61 0.0 0.0 0.0 19.300577792664544 0.0 62 0.0 0.0 0.0 19.851678948249873 0.0 63 0.0 0.0 0.0 20.564499246357393 0.0 64 0.0 0.0 0.0 21.215562719812425 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGCCG 15 0.0022186318 70.02723 38 CTATCGG 20 0.00693972 52.506676 27 GCGAAGT 20 0.00693972 52.506676 32 CCATATT 20 0.00693972 52.506676 32 TCCGTGC 20 0.00693972 52.506676 36 TTCGGTA 20 0.00693972 52.506676 58 TGCCGTA 20 0.00693972 52.506676 40 GTGCCGT 20 0.00693972 52.506676 39 GAATAAC 20 0.0069469092 52.49293 65 CTTTGCG 20 0.0069469092 52.49293 24 GCCGTAT 275 0.0 43.278233 54 TCGCCGT 255 0.0 41.1817 52 CGCCGTA 275 0.0 40.732452 53 CAACAGT 35 0.001252359 40.005085 36 CCGTATC 280 0.0 40.005085 55 GCGCTGG 35 0.001252359 40.005085 15 CTCTAAC 35 0.001252359 40.005085 1 GTAGATC 290 0.0 39.84308 38 CGTATCA 300 0.0 39.67171 56 GTCGCCG 265 0.0 39.61731 51 >>END_MODULE