FastQCFastQC Report
Wed 25 May 2016
SRR1780866_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780866_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191072
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT158928.317283537095964No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26461.384818288393904No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCG26021.361790319879417No Hit
ATTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT18260.9556606933511975No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCC14440.7557360576117903No Hit
CTTAAACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT6610.3459428906380841No Hit
ATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGTCTT5210.2726720817283537TruSeq Adapter, Index 8 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCC4350.22766287054094791No Hit
CTTATACACATCTCCGCGCCCACGAGACCATTTCGAATCTCGTATGCCGT2900.1517752470272986No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACCA150.002223541769.9882231
ACTCTCG207.918329E-569.9882236
CGATTTG150.002223541769.9882232
GGGACCG150.002223541769.988225
AGGGGGG22400.062.36590670
AAACTCG401.0622898E-861.23969736
ATATCGA352.9662078E-759.98990630
TGCCGAC352.9662078E-759.98990645
CGTCTAC308.348854E-658.33878748
AGCCGTC308.361823E-658.32351745
CCGACTT308.361823E-658.32351747
CGACTCT308.361823E-658.32351734
CTCTCGT308.361823E-658.32351737
GCCGTAT308.361823E-658.32351746
ATCGTAT553.6379788E-1157.2630939
ATATCGT553.6379788E-1157.2630937
ACCTGCG252.3879607E-455.990587
AATATCG509.767973E-1055.9905836
AAGCCGT252.3879607E-455.9905844
AACTCGT452.6911948E-854.43528737