FastQCFastQC Report
Wed 25 May 2016
SRR1780865_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780865_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315877
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG299039.466659490877777No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG13000.4115525980049196No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCGA252.388159E-456.00101559
GGTACAT200.00694689552.500951
TACCCGC200.00694689552.5009511
CTCGGTG2850.049.13147744
GATCTCG2850.047.91077841
TCGCCGT2650.047.5480352
ATCTCGG2900.047.0772742
GCCGTAT2700.046.6675154
TACCGTT305.872368E-446.6675112
TCGGTGG2950.046.27202645
AGATCTC3000.045.51523640
GTCGCCG2850.045.4394251
CGGTGGT3100.045.1621146
TGGTCGC2850.044.21132749
GTAGATC3250.043.0913538
CCCGATC659.984433E-943.077733
CGCCGTA3000.042.0007653
TAGATCT3350.041.8050439
TCTCGGT3300.041.37093743
CGAGTTC350.001254274940.00072560
Produced by FastQC (version 0.11.5)