FastQCFastQC Report
Wed 25 May 2016
SRR1780865_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780865_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315877
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGT146014.6223688334383315No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24280.7686536215045731No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCG23420.7414278342519398No Hit
ATTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGT17510.5543296916204725No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCC16810.5321691671125153No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGCCCGAATCTCGTATGCCGT8470.2681423465462822No Hit
CTTAAACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGT5780.18298261665141813No Hit
ATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGTCTT5210.1649376181235101RNA PCR Primer, Index 40 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCC3590.11365183283366627No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAATC600.064.15694433
CGAAACT501.4551915E-1162.99045634
AGGGGGG26100.060.3547970
CGAATGC700.060.00040441
TCGGATA252.3882934E-456.0003837
AACTCGT509.786163E-1056.0003837
GACGACT252.3882934E-456.0003846
AAACTCG700.055.00037436
TGACGTC1100.054.09127845
TGCCCGA1950.053.84651630
GAAACTC653.6379788E-1253.83799735
GCTTTAC200.006938508252.5169871
AAGCCGT1000.052.5003644
CATCTCG608.367351E-1152.50035536
CACCCGT200.00695155752.49204624
CGTCTTC29850.051.59164448
AATCTCG33750.051.22997736
CCGTCTT29950.051.18565447
ATCTCGT33700.051.0982737
CTCGTAT33600.051.04201539