FastQCFastQC Report
Wed 25 May 2016
SRR1780864_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780864_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266075
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2922410.98336935074697No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG11900.44724231889504845No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACA150.002223517569.9960529
CCGTGAC150.002223517569.9960528
CATCGTG150.002223517569.9960528
TCTGCAC200.006942579552.506913
CGGAAAG200.006947743752.4970429
TAGACCG200.006947743752.4970414
CGGTTGA200.006947743752.4970429
CCGTCGT200.006947743752.4970411
ATCGTGA200.006947743752.4970429
TCGCCGT2150.052.09008452
GCCGTAT2100.051.66375454
GTCGCCG2300.050.2145651
CGGTGGT2100.050.0065846
CGTATCA2200.049.31540356
TGGTCGC2300.048.7020649
CTCGGTG2150.047.21551544
TCGTTGT305.872846E-446.66403234
CATCGTT305.872846E-446.66403232
GATCTCG2300.045.6581841
TCGGTGG2300.044.13624245
Produced by FastQC (version 0.11.5)