FastQCFastQC Report
Wed 25 May 2016
SRR1780864_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780864_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266075
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCGT155005.8254251620783615No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCG24660.926806351592596No Hit
ATTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCGT23560.8854646246359109No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21810.819693695386639No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCC10690.40176641924269474No Hit
CTTAAACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCGT6020.22625199661749507No Hit
CTTATACACATCTCCGCGCCCACGAGACCGCCGTGCATCTCGTATGCCGT3880.14582354599267125No Hit
ATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCGTCTT3590.13492436343136333RNA PCR Primer, Index 16 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCC2840.10673682232453256No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGT150.00222148170.0122159
TAGCGTT150.00222148170.01221510
TGACGTA150.002223138269.9990645
AGGCCGT150.002223138269.9990668
CGGTCTA150.002223138269.9990637
CGTTTAG150.002223138269.9990613
GCCGTAA207.917963E-569.9990646
AGGGGGG24300.062.79751270
GATCCTA252.3878449E-455.9992451
AGACGTG252.3878449E-455.99924529
CGTAAGA252.3878449E-455.99924541
TAAGACG252.3878449E-455.99924543
CGTAATC252.3900642E-455.9887248
CATCTCG31350.053.0295936
ATCTCGT31600.052.72080637
GCCGTCT29100.052.67970746
CCGTCTT28850.052.6509447
TCGTATG32150.052.5809440
CGTCTTC29100.052.54955748
CAGACGT200.00694656252.499328