FastQCFastQC Report
Wed 25 May 2016
SRR1780863_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780863_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333282
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG261177.836306791245852No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG13410.4023619637424163No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT3650.050.8293354
CGCCGTA3700.050.14244553
CGGTGGT4100.048.6583346
CGTATCA3900.048.46860556
TGGTCGC3800.046.97348449
CCGTATC4050.046.6734755
GGTGGTC4100.046.09736347
TCGGTGG4250.045.29392245
GTGGTCG4100.045.2437148
TCTCGGT4400.044.55195243
TCGCCGT4050.044.08049852
GTAGATC4250.042.82334538
GTATCAT4350.042.649957
AGATCTC4200.042.49981740
CTCGGTG4400.042.16523744
GATCCTA757.530616E-1042.0061261
TAGATCT4250.041.99981739
ATCTCGG4350.041.838942
GTCGCCG4700.037.97286251
TATCATT4700.037.2394758
Produced by FastQC (version 0.11.5)