FastQCFastQC Report
Wed 25 May 2016
SRR1780863_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780863_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333282
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT140274.2087481472146715No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCG25020.7507156102039715No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24860.7459148708901171No Hit
ATTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT17560.5268811396955131No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCC14280.42846598376149925No Hit
CTTAAACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT5570.167125737363554No Hit
ATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGTCTT4760.14282199458716643RNA PCR Primer, Index 45 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC150.002224232569.99433
AGGGGGG20500.061.8276870
ATCGTAA200.006945842452.503639
TCGTATG27600.050.98176240
TATGCCG26600.050.9169143
CGTATGC27400.050.71515741
CCGTCTT25700.050.65735647
CGTCTTC26300.050.56622348
CTCGTAT27300.050.5162939
ATGCCGT26700.050.33297744
CTGCTTG26100.050.3059154
TCTCGTA27600.050.21330338
GCCGTCT26350.050.2046446
TGCCGTC26700.050.20190445
ATCTCGT27500.050.01410737
GTATGCC27350.049.64860542
CTTCTGC27150.049.51358851
GTCTTCT26900.049.4383549
TCTTCTG27000.049.25524550
CTTGAAA27800.049.23738557