FastQCFastQC Report
Wed 25 May 2016
SRR1780862_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780862_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387025
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG262816.7905174084361475No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG15100.39015567469801693No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTG150.002223525470.0020764
GCTTGTG200.006940657752.5151251
GTCTAGA200.00694420752.508342
TCGCGTT200.00694775752.5015563
GCGTGTA200.006951309352.49476634
CGGTGGT2250.049.79211846
TGGTCGC2100.048.3472649
GCCGTAT2150.047.210754
TCGGTGG2450.045.72745545
GTCGCCG2250.043.5568451
CTCGGTG2350.043.19834544
GTAGATC2400.042.2874538
TCGTTCG503.480358E-641.99038723
TCGCCGT2350.041.70335852
CGTATCA2700.038.89506556
CATTCGG459.181992E-538.8950644
TCTCGGT2650.038.30797243
ATCTCGG2650.038.2980742
GATCTCG2650.038.2980741
TTCGGTA556.6982157E-638.1730826
Produced by FastQC (version 0.11.5)