FastQCFastQC Report
Wed 25 May 2016
SRR1780862_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780862_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387025
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGT150333.884245203798204No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCG27370.7071894580453459No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26970.6968542083844713No Hit
ATTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGT16240.4196111362315096No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCC16240.4196111362315096No Hit
CTTAAACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGT7340.1896518312770493No Hit
ATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGTCTT4330.11187907757896777RNA PCR Primer, Index 15 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT150.002223460570.002594
TTAGACC150.002224600469.9935449
GCCGTAT150.002224600469.9935446
AGGGGGG23950.060.48764470
CGAATGC553.6379788E-1157.2674441
CGTCTTC28600.054.69774648
TCGTATG29800.054.4916240
CGTATGC29350.054.37317741
TATGCCG28750.054.28362743
GCCGTCT28700.054.14134246
ATGCCGT29200.054.0532344
CCGTCTT28500.054.03009847
CTCGTAT29800.053.9113939
TCTCGTA29800.053.9113938
ATCTCGT29850.053.82108337
GTATGCC29050.053.72303842
TGCCGTC29400.053.44744545
GTCTTCT29850.053.2279249
TCTTCTG30100.052.78582450
CTTCTGC29850.052.53804451