FastQCFastQC Report
Wed 25 May 2016
SRR1780861_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780861_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410531
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG274596.688654449968455No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG12240.2981504441808292No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT200.006950733752.49643733
TACCGAT200.006950733752.49643724
ATCGTAT200.006954083752.49004430
TGGTCGC1850.045.4078649
TCGCCGT1850.043.51586552
CGGTGGT2200.039.7700346
GCCGTAT1950.039.484554
GTCGCCG2050.039.27041251
CTCGGTG2250.038.89098744
TCGGTGG2300.038.04553245
TCTCGGT2450.037.1448643
ATCTCGG2400.035.0018942
CAGTTAC400.00241698735.001891
TGGAACC400.00241698735.001894
AGATCTC2400.034.99762740
GTAGATC2400.034.99762738
GATCTCG2400.034.99762741
AAATTCG400.002418432234.99762340
CGCCGTA2250.034.22406853
GGTGGTC2700.033.7055247
Produced by FastQC (version 0.11.5)