FastQCFastQC Report
Wed 25 May 2016
SRR1780861_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780861_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410531
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCGT134913.2862317340225218No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCG25390.6184673021038607No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23330.5682883874786557No Hit
ATTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCGT19050.46403316680104545No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCC13890.33834229327383314No Hit
CTTAAACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCGT5900.14371630887801407No Hit
ATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCGTCTT4300.10474239460601027RNA PCR Primer, Index 31 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGT150.002222286870.01266537
AGGGGGG22650.057.6273470
TAACTCG407.4631316E-752.50950636
AGCCGTC407.475701E-752.4967145
TACGCTC200.006950590852.4967141
ATACGAT200.006950590852.4967124
AATACGA200.006950590852.4967123
CCGTCTT24900.051.7299747
GCCGTCT25500.051.61089746
CGTCTTC25100.051.5966848
TATGCCG26600.051.56943543
CGTATGC26950.051.29548641
TCGTATG27750.051.20440
TCTCGTA28050.050.91830438
ATGCCGT26650.050.8223344
CTCGTAT27850.050.7811839
ATCTCGT28200.050.771637
GTATGCC27100.050.6179742
TGCCGTC26950.050.12672845
GTCTTCT25650.050.08093649