FastQCFastQC Report
Wed 25 May 2016
SRR1780860_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780860_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382699
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG279847.312274137115591No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG13280.3470090070786702No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTCC200.00694794752.5010811
CGTTCCT200.00694794752.5010812
CATTTCG200.00695153952.49421725
GCCGTAT2300.044.13134454
TCGCCGT2400.042.29253852
TGGTCGC2600.041.72617349
CACGGTA350.001253786140.0060567
CGCCGTA2700.038.88968753
GTCGCCG2700.038.88460551
CTCGGTG2600.037.6930844
CGTATCA2750.035.63709656
TCGGTGG2950.035.589345
GATCTCG2400.035.0007241
CCGGACG400.002417180235.00071735
ATCTCGG2450.034.2864242
CGGTGGT2900.033.78938346
TCTCGGT2800.033.75069443
CCGTATC3050.032.1318155
GGTCATA552.9893903E-431.8188361
GTAGATC2900.031.37995538
Produced by FastQC (version 0.11.5)