FastQCFastQC Report
Wed 25 May 2016
SRR1780860_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780860_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382699
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT151103.94827266337252RNA PCR Primer, Index 14 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCG27230.7115252456891709No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25750.6728525551412468No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCC18140.47400176117523174RNA PCR Primer, Index 14 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT16870.44081641185370224No Hit
CTTAAACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT6470.16906237016558706RNA PCR Primer, Index 14 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGTCTT4830.1262088482070766RNA PCR Primer, Index 14 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCG352.972265E-759.99540736
AGGGGGG23150.059.73026770
CCGACTT252.3914617E-455.988447
GTCGGAC200.006947022452.50284211
CGTCTTA750.051.32940348
ATCTCGT30150.050.84187737
GCCGTCT28300.050.8198646
CCGTCTT28100.050.80797647
TATGCCG29350.050.79015743
CGTCTTC28150.050.60003348
CATCTCG30650.050.24085236
CTCGTAT30100.050.22871439
CGTATGC29900.050.21354741
GTATGCC29700.050.19161642
TCTCGTA30550.050.0616338
TGCCGTC29850.050.05662545
CTTGAAA28900.050.02000857
GTCTTCT27750.049.9421249
TCGTATG30750.049.8498440
TCTTCTG27900.049.81207350