FastQCFastQC Report
Wed 25 May 2016
SRR1780859_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780859_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108694
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2695524.798976944449556No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG17551.6146245422930428No Hit
TCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT2070.19044289473199993No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC1450.1334020277108212Illumina Single End PCR Primer 1 (95% over 21bp)
CTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG1450.1334020277108212No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1120.10304156623180671No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCATCG150.002219471470.026
GTGGGTC150.002219471470.038
CGTTCAA150.002219471470.035
TGTGCAC150.002219471470.053
ACGTGTC150.002219471470.064
ATGCAAC150.002219471470.013
AGTACGT150.002219471470.061
GTACGTG207.8964E-570.062
TACGTGT150.002219471470.063
TACCCGC252.3813809E-455.99999611
GTCTCGG252.3813809E-455.99999642
TGCGGGT252.3813809E-455.9999965
CTACCCG252.3813809E-455.99999610
TCTCGGT3100.053.06451443
TCGCCGT2800.052.552
AGCGTTC200.006935191352.533
CGCGGCG200.006935191352.57
GCGCGTC200.006935191352.56
CGTGATG200.006935191352.531
TCCTCGT200.006935191352.512
Produced by FastQC (version 0.11.5)