FastQCFastQC Report
Wed 25 May 2016
SRR1780859_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780859_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108694
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGT1576614.504940475095221No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30162.7747621763850807No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCG28492.6211198410215837No Hit
ATTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGT15741.4481020111505694No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCC15331.4103814377978545No Hit
CTTAAACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGT7600.6992106280015455No Hit
ATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGTCTT5150.4738072018694684RNA PCR Primer, Index 38 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCC3370.31004471267963274No Hit
CTTATACACATCTCCGCGCCCACGAGACATTTATCTATCTCGTATGCCGT3070.2824442931532559No Hit
CTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGTC1490.13708208364767147No Hit
ATTAAACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGT1270.11684177599499512No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTCG150.002219471470.058
TCACCGA207.8964E-570.011
TAGGTTC150.002219471470.043
GGCAATT150.002219471470.019
GACAACG150.002219471470.018
TTGCCGG150.002219471470.010
CGTTTGG207.8964E-570.028
AGGGGGG22100.063.981970
CGGATCA308.329345E-658.3333329
ACGGATC308.329345E-658.3333328
GGACGGA308.329345E-658.3333326
CAGTCAT308.329345E-658.33333247
CGACTTC308.329345E-658.33333248
TAACTCG308.329345E-658.33333236
CGTATGA850.057.6470641
ATGACGT850.057.6470644
ATCAGAC252.3813809E-455.99999612
AGGACGG252.3813809E-455.9999965
TAAACAC2000.055.9999963
ATCACCG252.3813809E-455.99999610
Produced by FastQC (version 0.11.5)