FastQCFastQC Report
Wed 25 May 2016
SRR1780858_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780858_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166486
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2746316.49568131854931No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG1910.11472436120754897No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCGG150.002220229770.0105110
ATTTCGC150.00222287869.9894928
TTTCGCC207.9146324E-569.9894829
CGAGTAA200.00693752452.50788510
TGGAACC200.00693752452.50788518
TGCGCGA200.00693752452.50788510
TTTCGAC200.00694577552.4921128
TGGCGTA200.00694577552.4921124
TCGCCGT503.459334E-642.00631352
TGGTCGC503.4664936E-641.9936949
CGCCGTA556.657883E-638.18755753
CGGTGGT556.6716384E-638.17608346
TCGGTGG556.6716384E-638.17608345
AAAACGT400.002411734835.00525714
GTAAGCG400.002411734835.00525757
GCCGTAT601.2090713E-535.00525754
TCTCGGT601.2115646E-534.99474343
CCCACGT400.002415293634.9947470
TCAGCAA400.002415293634.9947430
TGTACGG400.002415293634.994745
Produced by FastQC (version 0.11.5)