FastQCFastQC Report
Wed 25 May 2016
SRR1780858_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780858_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166486
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGT161249.68489842989801No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCG21141.2697764376584217No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCC14140.8493206635993418No Hit
CTAATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGT6580.3952284276155352No Hit
CTTAAACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGT6000.36039066347921145No Hit
CATATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGT5630.3381665725646601No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3770.22644546688610456No Hit
ATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGTCTT3670.22043895582811768TruSeq Adapter, Index 1 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCC2480.14896147423807407No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTCAGCGATCTCGTATGCCGT2450.14715952092067802No Hit
CTTATACACATCTCCGCGCCCACGAGACTGTCCGCGATCTCGTATGCCGT2100.126136732217724No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTA150.002220418870.0090115
CAGTCCG353.6834535E-970.0090128
TAGCGTG150.002223067269.9879812
CCGCGAA353.6925485E-969.9879832
CGTCTTA550.063.64455848
TGCCGAC501.4551915E-1162.9891945
AGGGGGG22650.062.8999370
AGTCCGC453.8198777E-1062.2115429
CGCGAAC401.0586518E-861.2578933
CCGACTT452.6820999E-854.45145847
AAGCCGT452.6884663E-854.43509744
GCCGACT452.6884663E-854.43509746
ATCTCGA653.6379788E-1253.8530937
CGTATGA653.6379788E-1253.8369141
ATCGTAT850.053.5363139
ATATCGT850.053.5363137
TCTTCGT200.00693811152.5067620
CGTTGAT200.00693811152.5067616
AAGACGA200.00693811152.5067652
GCGTTGA200.00693811152.5067615