FastQCFastQC Report
Wed 25 May 2016
SRR1780857_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780857_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458274
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG319746.977048665209024No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCCGG150.0022236570.0031618
GCCTTTA601.2145774E-535.001581
CCGTGCG601.2154858E-534.9977659
CCCGTGC601.2154858E-534.9977658
CGTGCGA652.1021953E-532.3056310
CTATGTT450.004304832331.11251632
CCTTGCG450.004304832331.11251654
GAACCCG450.004307131731.10912320
GCCGTAT703.4852354E-530.00135654
TAGCCCG1202.2955646E-929.1648035
CGAGAAT853.950776E-628.82483314
CGTGGAA500.00720432728.00126612
TTTCCGG500.00720432728.00126632
GTTACCG500.00720432728.00126618
TCGCCGT755.5791254E-528.00126552
AACGTGG755.5791254E-528.00126515
GAGAACG755.5791254E-528.00126512
CGTGCTT500.007208165727.99820910
ACATTCG500.007208165727.99820911
AAGTTCA500.007208165727.99820925
Produced by FastQC (version 0.11.5)