FastQCFastQC Report
Wed 25 May 2016
SRR1780857_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780857_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458274
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGT181273.955493874843434No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCC22930.5003556824083408No Hit
TCTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCG22130.48289887709099794No Hit
CTTAAACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGT7230.15776587805548645No Hit
CTAATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGT7040.15361988679261754No Hit
CATATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGT6480.1414001230704775No Hit
ATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGTCTT4670.10190410103998918Illumina PCR Primer Index 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACAT150.002222948270.008731
AGCGTTG150.002223910570.001099
AACTCGT207.922925E-570.0010937
AGGGGGG27350.061.04966470
CGAATGC700.059.99438541
GATCTTA308.365896E-658.340611
CGTATGA1450.057.92561741
TGACGTC1100.057.2736245
AAGCCGT452.6984708E-854.44529344
AGTGACG653.6379788E-1253.8411230
TCTTCTG36050.052.7250250
GATCTCG35450.052.6242336
ATCTCGT35000.052.60082237
CGTCTTC36350.052.5787748
CGATCTC35150.052.575535
TGCCGAC200.00694895452.50081645
GCCGTCT35700.052.4575246
CCGTCTT35300.052.45696647
ACGATCT34650.052.3240534
GACGATC35450.052.03183733