FastQCFastQC Report
Wed 25 May 2016
SRR1780856_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780856_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281742
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3518412.488020955342122No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG3110.11038467818074692No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCGGT150.002222417670.0058611
TCGCCGT800.056.86966352
ATCGCAG252.3848466E-456.01463340
GCCGTAT850.053.5243954
GGTACGG200.00693943952.513721
CGAGGAC200.00694431552.504449
TGCGAAT200.006949193352.49507523
CAATACG200.006954074452.4857610
TTAAACG305.8642443E-446.67886414
TGGTCGC900.046.6705749
AGATCTC950.044.2220840
GATCTCG950.044.2220841
CGGTGGT950.044.2142346
GTAGATC1100.041.38179438
TTTCAAG350.001252544540.01045236
ATCTCGG1050.040.0033542
CTCGGTG1050.040.0033544
TCGGTGG1050.040.0033545
GAATTTC350.001254734139.99624651
GTCGCCG1250.039.19632351
Produced by FastQC (version 0.11.5)