FastQCFastQC Report
Wed 25 May 2016
SRR1780856_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780856_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281742
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGT206937.344662847569762No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCG28421.008724293857501No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCC20870.7407486281775525No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGT7440.2640713844581212No Hit
CTAATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGT7200.2555529527014077No Hit
CATATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGT6400.22715818017902903No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5980.21225092460478026No Hit
ATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGTCTT4150.14729788245983916Illumina PCR Primer Index 4 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCCGT301.02136255E-770.0012444
GGAACTT150.002224565369.98881559
CGAATGC600.064.1564241
AGGGGGG29850.062.62529470
CGTATGA950.058.9379541
AACTCGT601.8189894E-1258.3343737
CGTCTTA252.383532E-456.0208848
CGTCTAC252.383532E-456.0208848
TGACGTC1000.056.0109445
AAACTCG700.055.0009836
TGCCGAC452.6897396E-854.45508245
GCCGTCT36950.053.33923746
CCGTCTT36700.053.32103747
CGTCTTC37450.053.1039148
AATCTCG37150.053.04266436
GTCTTCT37300.052.8481249
TCTTCTG37350.052.77737450
ATCTCGT37000.052.59552837
CTTGAAA38300.052.537357
CTGGTAA200.00694125352.51025453