FastQCFastQC Report
Wed 25 May 2016
SRR1780855_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780855_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341811
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG299788.770343844990359No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGTCC150.002224614469.9916650
ACCAGCG150.002224614469.991668
CAGTAGT252.3883315E-456.0015221
GATCATA200.006947133752.5014231
AGCGCAT200.006951154752.49374451
GGTCATA352.084034E-550.0013581
TGGTCGC1000.042.0011449
TCGCCGT757.548806E-1041.99499552
TCGGTGG953.6379788E-1240.52741645
GCTTCTA801.4188117E-939.376071
CGGTGGT1100.038.18285846
CTCGGTG1051.4551915E-1136.66766444
GCCTTTA400.002416741835.000951
TCAGCGC400.002416741835.0009549
GTGCCCC400.002416741835.0009558
GCCGTAT904.494723E-934.99583454
TCGCCAC552.9886104E-431.81904632
AGCCTTA552.991177E-431.8143926
CATGGGT450.004306777431.10740756
CCTCGTT450.004306777431.1074078
Produced by FastQC (version 0.11.5)