FastQCFastQC Report
Wed 25 May 2016
SRR1780855_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780855_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341811
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT170134.977312023311128No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCG22570.6603064266509855No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCC18040.5277770463794319No Hit
CTTAAACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT6890.20157338412163447No Hit
CTAATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT6500.19016356992607025No Hit
CATATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT5920.17319512830189782No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4810.14072104174529199No Hit
ATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGTCTT3420.10005529371494774RNA PCR Primer, Index 15 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTAGT301.0185795E-770.0352861
GCCGACT455.456968E-1270.0045446
CCGACTT353.7016434E-970.0045447
CTTTATA353.7016434E-970.004542
AACTCGT401.3460522E-1070.0045337
ATCTGCA150.00222428269.994329
AGACGAT207.929605E-569.98405525
AAACTCG501.4551915E-1163.00408636
CGACTTC453.8380676E-1062.21714848
AGGGGGG26750.060.9759170
GGGTCTA352.9594048E-760.030241
GACAATA308.3425875E-658.3627361
TGCCGAC601.8189894E-1258.32858345
GAATTAC252.3827112E-456.0282251
TATGCCG32800.053.3493143
CGTATGC33200.053.3468341
TCGTATG33550.053.20762340
CGTCTTC31800.053.1560448
GCCGTCT32100.053.10313446
CCGTCTT31600.053.05723647