FastQCFastQC Report
Wed 25 May 2016
SRR1780854_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780854_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259223
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3136712.100392326298207No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAACCG150.002223271969.9974931
GGCGGTC150.002223271969.9974944
GGTCTCG352.0834023E-549.9982141
GCCGTAT506.168921E-849.007754
GTCTCGG305.8719714E-446.66499342
TGGTCGC604.9167284E-946.66499349
TCGGTGG851.8189894E-1245.29249645
CGCCGTA602.586239E-740.8397553
GGTGACG350.001253001640.00628754
GCATGCC350.001254191239.9985711
GTCGCCG801.4169927E-939.37359251
TCGCCGT556.671955E-638.18781752
TCTCGGT1100.038.1804543
ATCTCGG753.5252015E-837.33199742
CTCGGTG1051.2732926E-1136.66535644
GTGACGG400.002414127635.005555
CTTTCTA400.00241641434.9987530
GATCTCG806.2213076E-834.9987541
GCTTCTA652.0982912E-532.3065341
CTTGAGG652.0982912E-532.30653445