FastQCFastQC Report
Wed 25 May 2016
SRR1780854_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780854_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259223
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT180716.971217831751041No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCG24220.9343306728183842No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCC18110.6986262793039197No Hit
CTTAAACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT7150.27582429028288385No Hit
CTAATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT6400.24689167242104287No Hit
CATATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT5930.22876056522762256No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4670.18015376721972973No Hit
ATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGTCTT4390.16935225655130912TruSeq Adapter, Index 8 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCC3380.13038966449736328No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAATC252.8356208E-669.9965333
ACGAGTG150.002223393269.9965238
AGGGGGG23000.064.6831870
CGTCTTA600.064.1634848
TTACGAA308.36433E-658.33043731
TTTCGCA509.786163E-1055.99722331
ATTACGA252.3881572E-455.99722330
CGTCTAC252.3881572E-455.99722348
AAACTCG452.6937414E-854.44174636
CAGCTTG1100.054.0986654
GGAATAT200.00694205752.5075261
TCAATGT200.006947356352.497433
TTGGGAA200.006947356352.497445
TTCGCAT552.2755557E-950.90656332
CGTATGC33150.050.78179641
ATCTCGT33050.050.61776437
GCCGTCT31100.050.52803846
TATGCCG32700.050.51737243
CCGTCTT30900.050.4020347
CTCGTAT33500.050.2512439