FastQCFastQC Report
Wed 25 May 2016
SRR1780853_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780853_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413072
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG327017.916537552775303No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCTC200.006946856652.50390665
TAGTCGA200.00695018452.4975539
TAGCTCG556.690936E-638.18003519
GATCATA400.002416763535.00261
TGCCCGT400.002418199334.99836741
GACGTTG501.7090593E-434.99836730
TGCGATG400.002418199334.99836712
ATCGAGG400.002418199334.99836714
AGCTCGT601.2150524E-534.99836320
GCTCGTC601.2150524E-534.99836321
TAATCGA708.685165E-734.99836321
AATCGAT751.4893158E-632.66514222
GTTTGAC552.9909785E-431.81669612
CATCGAG450.004306280531.1096613
TCGGTGG703.4837183E-530.0022345
GCCGTAT703.4837183E-530.0022354
TGACGTT604.980192E-429.16530429
TTTTCCG604.980192E-429.16530431
GATCCTA1352.582965E-1028.520641
CGGTGGT755.5767043E-528.0020846
Produced by FastQC (version 0.11.5)