FastQCFastQC Report
Wed 25 May 2016
SRR1780853_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780853_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413072
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGT171904.161502111012124No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCG23740.574718208932099No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCC21790.527510942402293No Hit
CTTAAACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGT6750.16340976875702057No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGCCCGAATCTCGTATGCCGT6450.15614711236781967No Hit
CTAATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGT6170.14936863307123213No Hit
CATATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGT5760.13944300267265755No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4650.11257117403261417No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACT550.063.63317546
TGCCGAC501.4551915E-1162.9968445
AGGGGGG26900.061.41699670
CCGACTT650.059.227847
CGACTTC601.8189894E-1258.33040648
GCCGTAT509.804353E-1055.99719246
CGTCTTA1300.053.84345248
TCGTCTC200.00695027852.49736828
TTGCGGC200.00695027852.49736834
TCCGTTT200.00695027852.49736849
ATGACGA200.00695027852.49736844
CGTATGC35750.051.8855141
CCGTCTT32550.051.82535647
TATGCCG35300.051.7537843
ATCTCGT36050.051.64789637
TCGTATG36500.051.58645640
AATCTCG35900.051.5712336
TCTCGTA36350.051.51048338
CTCGTAT36250.051.45948839
CGTCTTC32150.051.38155748