FastQCFastQC Report
Wed 25 May 2016
SRR1780852_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780852_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261908
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3228012.324938528032744No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAA451.681532E-646.66361250
TGCCGAG404.5868197E-543.74713557
TTGTACG350.001253220640.005029
GTTGTAC350.001253220640.005028
TGTAACG350.001253220640.0050212
GTAACGA350.001253220640.0050213
TTTCGTC350.00125439839.99738328
CGAGTTC501.7056268E-435.00439560
TGGTCGC501.707543E-434.9977149
TCGCCGT400.002416811934.99770752
CCTCACG652.0960595E-532.31174559
CGGGTAG552.984993E-431.82217210
CCGAGTT552.984993E-431.82217259
GCGGGTA552.984993E-431.8221729
GTAGATC552.9883397E-431.816138
TTTGCAT450.00429980631.11501517
TTGCATT450.00429980631.11501518
GCCGTAT450.004303826531.10907654
ACTACCG604.9758225E-429.16475770
TGCTAGC500.00719595428.00351511