FastQCFastQC Report
Wed 25 May 2016
SRR1780852_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780852_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261908
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCGT181716.937932403744826No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCG24200.9239885761412405No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCC15700.5994471341081601No Hit
CATATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCGT7090.27070574400171055No Hit
CTTAAACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCGT6560.25046963055729493No Hit
CTAATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCGT6410.2447424286390641No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4230.16150709409410938No Hit
ATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCGTCTT3660.13974372680483224RNA PCR Primer, Index 16 (95% over 23bp)
CTTATACACATCTCCGCGCCCACGAGACCGCCGTGCATCTCGTATGCCGT3080.11759854605433968No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACAT207.914318E-570.0051647
CGACTTA150.00222233270.0051548
ACGCCGT207.921809E-569.9917928
GGTTGGA207.921809E-569.991791
AGGGGGG27850.062.33852470
CGTCTTA453.8380676E-1062.22680748
CGTGAAT750.060.6595532
TGACGTC700.060.0044245
AATCTCG650.059.2238236
TGACGAC308.35846E-658.33762745
CGACGTG553.6379788E-1157.26600629
CGTATGA1000.055.9934341
AACGCCG252.3890022E-455.9934327
CCGCCGA750.055.9934328
GTGCAAC700.054.9935533
CAACTCG700.054.9935536
AGCCGTG1100.054.08456429
TCGTATG35850.053.2015740
TATGCCG35000.053.1937643
CGTATGC35350.053.1620841