FastQCFastQC Report
Wed 25 May 2016
SRR1780851_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780851_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382038
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG358319.378909951366095No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGAAT150.002222543370.0096914
CAGTAGT200.00694829652.5003931
GACCTAT200.00694829652.5003931
TTCCCAT305.8780215E-446.6609134
ACGGTTA551.3108001E-744.5399650
CGCGGGT404.5923633E-543.7446027
CGATAGA459.1828726E-538.8942737
TACGGAC601.2121849E-535.0094338
GATACCT601.215452E-534.9956828
TCGTGCC400.002418882434.9956831
GTCACAC501.70959E-434.9956848
CCAACGT400.002418882434.9956822
CGCTTGG400.002418882434.9956822
TGCTACC851.05932486E-732.9500538
GCCGTAT652.0983796E-532.31216454
ACTTATG652.1021411E-532.30370748
ACTGCTT552.9896092E-431.8184228
GGTCACA552.9919064E-431.81425747
ATTTCGT552.9919064E-431.81425728
TACGTAC450.004299224431.11949239
Produced by FastQC (version 0.11.5)