FastQCFastQC Report
Wed 25 May 2016
SRR1780851_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780851_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382038
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT214835.623262607384605No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCG31290.8190284736073374No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCC17100.44759945345750946No Hit
CTTAAACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT8000.2094032530795366No Hit
CTAATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT7140.1868924033734864No Hit
CATATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT6650.1740664541223648No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5790.1515556044163146No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGAC150.002224704569.9925437
AGGGGGG28450.062.39043470
CAACTCG352.9728653E-759.99360736
AACTCGT308.37472E-658.32711437
AGCCGTC509.786163E-1056.00136645
CTTTATA252.390268E-455.9940342
CGAATGC1150.054.78394341
CCGTCTT35850.052.9162147
TCTTCTG36350.052.67667850
CGTCTTC36950.052.67394348
GCCGTCT36500.052.6451246
GACGACA800.052.50127826
CGCATAG200.006951431352.4944045
ATCCGAC200.006951431352.4944043
TATGCCG36950.052.47759643
CGTATGC37700.052.4548641
ATCTCGT38050.052.42542337
TCGTATG38850.052.34361340
TCTCGTA38350.052.29592538
CATCTCG38650.052.24540336