FastQCFastQC Report
Wed 25 May 2016
SRR1780850_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780850_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361922
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG348979.642132835251795No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGGAC150.00222344370.0017919
ATCCCGG150.00222344370.0017913
GCGGACG150.00222344370.0017920
GGCGTGT150.00222466269.992137
AATCTCG200.00694750352.50134739
GTTGTGC200.00695130152.4940951
CGCTGCG305.8732927E-446.66786216
GCTGCGG404.587842E-543.7511217
GGAGTTC602.5955342E-740.8287428
TCCCGGC350.001253618340.00655435
GCGTGTG350.001255323439.99558
TCCGCTT459.188018E-538.8898931
CCGCTTC459.188018E-538.8898932
TCGGTCA556.6916637E-638.17752529
AACCGTA654.862104E-737.6880710
CTCGGTG852.568413E-937.05977644
CGGTGGT951.8735591E-1036.84305246
GGTCCAA708.6762884E-735.000911
AGGCATC501.7079148E-435.000921
GGTTCAC501.7079148E-435.000933
Produced by FastQC (version 0.11.5)