FastQCFastQC Report
Wed 25 May 2016
SRR1780850_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780850_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361922
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGT218116.0264366355181505No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCG32820.9068252275352148No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCC16970.4688855609772271No Hit
CTTAAACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGT8190.22629185294068885No Hit
CTAATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGT7890.21800277407839258No Hit
CATATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGT7520.2077795768148938No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6560.18125452445554566No Hit
ATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGTCTT4290.11853382773083702RNA PCR Primer, Index 15 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTTC500.070.00165648
TAAATCG150.0022234670.00165636
TGACGTC1000.066.5015745
ATCGTAT800.065.6265639
AGGGGGG30250.061.8937470
CGTATGA1200.061.25145341
CAAATGT352.969755E-760.0014228
CGTCTTA308.367364E-658.33471748
GCCGACT553.6379788E-1157.27408246
CAGCATG252.3885616E-456.00132454
CTTTATA653.6379788E-1253.8399932
CGTCTTC36750.053.6203248
TCGTATG38800.053.49353840
TCTTCTG37250.053.46435550
ATCTCGT37950.053.4004737
CTCGTAT38000.053.33020839
CCGTCTT36100.053.32536347
GCCGTCT36500.053.22043646
TCTCGTA38150.053.2122738
CTTGAAA38500.053.18308357