FastQCFastQC Report
Wed 25 May 2016
SRR1780849_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780849_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186823
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2858815.30218442054779No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG2360.12632277610358467No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTTT207.905127E-570.0109837
CCGTATT150.00222295869.9922414
CCTTGAT150.00222295869.992241
CTAGTGC200.006938664752.50823236
TACTCGC200.006938664752.50823220
GCGGGAC200.006946016652.494183
TCCGTAT200.006946016652.4941813
GACTGCG200.006946016652.494182
TACCGTG200.006946016652.494187
ACTCGAG305.8621605E-446.67398562
TCAACCG305.8621605E-446.67398532
TCAATCG305.869905E-446.66149547
GCGGGTG305.869905E-446.6614958
GAAGTGC305.869905E-446.6614956
CAATCGC305.869905E-446.66149548
GCCGTAT451.6794475E-646.66148854
CTCGGTG551.3058525E-744.54051644
CGGTGGT551.3058525E-744.54051646
TCGGTGG551.3058525E-744.54051645
AACCGCC350.001252104540.00627534
Produced by FastQC (version 0.11.5)