FastQCFastQC Report
Wed 25 May 2016
SRR1780849_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780849_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186823
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCGT158188.466837594942806No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCG22461.2022074369858102No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCC14230.7616835186245805No Hit
CTTAAACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCGT6440.3447113042826633No Hit
CATATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCGT6180.3307943882712514No Hit
CTAATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCGT5070.2713798622225315No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4740.2537160842080472No Hit
ATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCGTCTT3510.18787836615406026RNA PCR Primer, Index 31 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACCGAGATATCTCGTATGCCGT3430.1835962381505489No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCC2120.11347639209305063No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG401.3460522E-1070.009136
TCGTAAT150.002220834170.009154
GCCGTAT301.0205622E-769.99036446
AGGGGGG21600.062.2469870
TGCCGTA352.9652256E-759.99174545
GCCGTCA1450.055.50959846
ACGTATG452.6844646E-854.45152340
CACGTAT452.6844646E-854.45152339
TGACGTC653.6379788E-1253.8387445
ATGCCGT28600.053.10457244
TGCCGTC28550.052.82985345
CGTCTTC27450.052.7796248
CTCGTAT28600.052.75161439
CGTATGC28800.052.74991241
CCGTCTT27600.052.74636547
TCGTATG28950.052.71842640
TATGCCG28850.052.64439843
TCTCGTA28700.052.56781438
GCCGTCT28050.052.5239646
TACCTGG200.00693205252.520897