FastQCFastQC Report
Wed 25 May 2016
SRR1780848_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780848_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634393
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG330145.204029678763795No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT150.00222465269.9983447
ACGTGCC200.006949090452.502969
TGCGCGT200.006953424352.4946255
GCCGTAT1100.044.55142254
GTGTGCG551.3108001E-744.5479132
TCGCCGT1200.043.75586352
GTCGCCG1200.043.74896651
TGTGCGC459.194244E-538.8910373
TCGGTGG1400.037.5020745
TGGTCGC1500.037.3324549
TGCGCGC400.002417963535.00193411
CGGTGGT1700.035.00193446
CTCGGTG1350.033.7082244
TCGTTCC552.993754E-431.81492229
GGACCTA450.004305856331.112831
TACCACG450.00430751831.11037820
ACGTTCA450.00430751831.11037820
GCTTGTG450.004309179731.10792529
TGCGCGG450.004309179731.1079255
GAACGTA450.004309179731.10792530
Produced by FastQC (version 0.11.5)