FastQCFastQC Report
Wed 25 May 2016
SRR1780848_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780848_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634393
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT191683.0214709178695225RNA PCR Primer, Index 14 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCG25170.39675721516473234No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCC22670.35734946634026543RNA PCR Primer, Index 14 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT7100.11191800666148585RNA PCR Primer, Index 14 (95% over 23bp)
CTAATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT6580.10372119490599674No Hit
CATATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT6350.10009568201414581No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCGA150.002220194370.033751
GCCGTAA352.9736475E-760.0005446
CGTCTAC308.37142E-658.3384648
TGCCGTA601.8189894E-1258.3338645
AGGGGGG29300.057.94481370
CGTCTTA800.056.87999348
ATATCGT1100.054.0871337
ATCGTAT1300.053.84239639
CCGTCTT33500.053.07510447
GCCGTCT33750.052.99307346
TATGCCG35450.052.8171943
CGTCTTC33650.052.63463648
GACGTAT200.006950360752.50047346
TGACGTA200.006950360752.50047345
ATGCCGT35600.052.40215744
CGTATGC36300.052.25530641
TCGTATG36750.052.18683240
TGCCGTC35150.052.17685745
TCTCGTA36000.052.10747538
ATCTCGT36050.052.03520237