Basic Statistics
Measure | Value |
---|---|
Filename | SRR1780848_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 634393 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT | 19168 | 3.0214709178695225 | RNA PCR Primer, Index 14 (95% over 23bp) |
TCTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCG | 2517 | 0.39675721516473234 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCC | 2267 | 0.35734946634026543 | RNA PCR Primer, Index 14 (95% over 21bp) |
CTTAAACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT | 710 | 0.11191800666148585 | RNA PCR Primer, Index 14 (95% over 23bp) |
CTAATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT | 658 | 0.10372119490599674 | No Hit |
CATATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT | 635 | 0.10009568201414581 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GGTCCGA | 15 | 0.0022201943 | 70.03375 | 1 |
GCCGTAA | 35 | 2.9736475E-7 | 60.00054 | 46 |
CGTCTAC | 30 | 8.37142E-6 | 58.33846 | 48 |
TGCCGTA | 60 | 1.8189894E-12 | 58.33386 | 45 |
AGGGGGG | 2930 | 0.0 | 57.944813 | 70 |
CGTCTTA | 80 | 0.0 | 56.879993 | 48 |
ATATCGT | 110 | 0.0 | 54.08713 | 37 |
ATCGTAT | 130 | 0.0 | 53.842396 | 39 |
CCGTCTT | 3350 | 0.0 | 53.075104 | 47 |
GCCGTCT | 3375 | 0.0 | 52.993073 | 46 |
TATGCCG | 3545 | 0.0 | 52.81719 | 43 |
CGTCTTC | 3365 | 0.0 | 52.634636 | 48 |
GACGTAT | 20 | 0.0069503607 | 52.500473 | 46 |
TGACGTA | 20 | 0.0069503607 | 52.500473 | 45 |
ATGCCGT | 3560 | 0.0 | 52.402157 | 44 |
CGTATGC | 3630 | 0.0 | 52.255306 | 41 |
TCGTATG | 3675 | 0.0 | 52.186832 | 40 |
TGCCGTC | 3515 | 0.0 | 52.176857 | 45 |
TCTCGTA | 3600 | 0.0 | 52.107475 | 38 |
ATCTCGT | 3605 | 0.0 | 52.035202 | 37 |