FastQCFastQC Report
Wed 25 May 2016
SRR1780847_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780847_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306057
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3465211.322073992752985No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG3670.11991230391724418No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTTT150.002222455670.0070319
GCCGTAT750.056.0056254
CGGTGGT750.055.99646846
TGTCCCG200.00694443252.5052710
GGTCGTC200.00694892152.496691
CGGTATG200.00694892152.4966943
CGTCCAA352.084631E-549.9968522
TCTCGGT950.047.89171643
AGTGCGG451.6805479E-646.671358
TCGCCGT900.046.66372752
GATCTCG1050.046.66372741
TGGTCGC900.046.66372749
CTTAAAG305.874431E-446.6637234
CTCGGTG1000.045.4971344
TCGTCCA404.588576E-543.74724221
GTCGCCG1050.043.330651
TCGGTGG1050.043.330645
ATCTCGG1150.042.60601442
AACCCTT350.001253706940.0040136
CCTTACG350.001254714839.997481
Produced by FastQC (version 0.11.5)