FastQCFastQC Report
Wed 25 May 2016
SRR1780847_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780847_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306057
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGT209246.836635005897594No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCG27740.9063671146224396No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCC25280.8259899299803631No Hit
CTTAAACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGT8560.2796864636325913No Hit
CTAATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGT7510.24537912872438797No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6720.21956694341250158No Hit
CATATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGT6540.2136856859996667No Hit
ATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGTCTT5430.17741793195385172RNA PCR Primer, Index 38 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCC3460.1130508369356035No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG27650.063.6787670
CGAATGC509.786163E-1055.99777641
TATGCCG36450.052.61794343
CGTATGT200.0069437952.50649628
CGTCTTA407.4682066E-752.49791748
CGTCTAC407.4682066E-752.49791748
CGTATGC37300.052.3571741
TCGTATG38400.052.3156340
GCCGTCT36200.052.30455446
CCGTCTT35800.052.30239547
GTATGCC36750.052.28363842
CTCGTAT38000.052.2216139
TCTTCTG36800.052.21260550
CGTCTTC36800.052.21260548
TCTCGTA37900.052.0823738
ATCTCGT37850.052.05869737
CTTGAAA38000.051.85320757
TGCCGTC36950.051.71648445
GTCTTCT37100.051.6960649
ATGCCGT37000.051.646644