FastQCFastQC Report
Wed 25 May 2016
SRR1780846_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780846_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences983714
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG193271.9646970562582213No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGA150.002223956470.0067749
TCGCCGT2300.041.08883352
TGGTCGC2450.040.0038749
CGGTGGT2700.038.89462746
GTCGCCG2400.037.91840451
GCCGTAT2500.037.7998154
TCGGTGG2700.035.0051645
CGCCGTA2700.035.00160253
CGTATCA2750.033.0907456
TCTCGGT2950.032.03862443
TACGAAC450.004310413331.10778823
CTCGGTG3050.030.98817444
CCGTATC3150.028.88874255
GTGGTCG3750.028.00270548
CCCCGTT500.007210050727.99985914
CGAATTG959.38901E-625.79196436
GGTGGTC4450.024.38561847
GTAGATC4050.023.33558838
TTTCGAC750.001831814223.33558769
TAACCGT750.001834524923.3296555
Produced by FastQC (version 0.11.5)