FastQCFastQC Report
Wed 25 May 2016
SRR1780846_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780846_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences983714
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGT65560.666453867689186No Hit
ATTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGT42040.42735998471100345No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19080.19395881323230124No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCG13160.1337787202377927No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC19200.053.40901641
CCGTCTT18050.053.32166347
TCGTATG19450.053.08240540
TATGCCG19000.053.0475143
CGTCTTC18300.052.97302248
ATGCCGT19100.052.5892244
GCCGTCT18650.052.3568546
TGCCGTC19200.051.9507545
TCTCGTA19150.051.72349538
ATCTCGT18750.051.70693237
CTCGTAT19500.051.69256639
GTATGCC20150.050.01998542
AGGGGGG17900.048.68739770
CCGCGAT20600.048.41765232
CGCGATC20050.048.1724533
GCGATCT20350.047.97818434
GATCTCG20600.047.91286536
CGATCTC20750.047.2243535
AAGTTCG305.8788405E-446.66690470
TCCGCGA22500.044.95121431
Produced by FastQC (version 0.11.5)