FastQCFastQC Report
Wed 25 May 2016
SRR1780845_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780845_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380476
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG224425.89840095038846No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG11430.30041316666491447No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTGA200.006942650352.51114358
CGAACTC200.00695348752.4904451
TCGCCGT2050.046.10128852
CTCGGTG2150.045.58507544
TCGGTGG2250.045.10882645
TGGTCGC2100.044.9977249
CGGTGGT2200.044.54319846
GCCGTAT2150.043.9570454
CGCCGTA2200.042.95801553
AGATCTC2300.041.08487340
GATCTCG2300.041.08487341
GTCGCCG2350.040.20544451
GTAGATC2400.039.37817838
GGTCCCA459.171702E-538.9022561
ATCTCGG2500.037.79808442
TCTCGGT2600.037.69535443
GGTCATA501.704888E-435.012031
TAGTCCA400.002418007234.99822630
TTTAGCG400.00241956734.99362626
TGTTCGT400.00241956734.99362610
Produced by FastQC (version 0.11.5)